Nephrin antibody (AA 1243-1256)

Catalog No. ABIN285873

Product Details anti-Nephrin Antibody

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Binding Specificity: AA 1243-1256

Reactivity: Human, Mouse

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Nephrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purity: Whole antiserum

Immunogen: Nephrin antibody was raised in guinea pig using a synthetic peptide corresponding to residues 1243-1256 of the intracellular domain of nephrin as the immunogen.

Isotype: IgG1

Application Details

Application Notes: IHC-F: 1:50, IHC-P: 1:50, WB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid with 0.09 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Storage: 4 °C

Storage Comment: Store at 4 °C

References for anti-Nephrin antibody (ABIN285873)

Zhou, Chen, Lu, Wu, Yuan, Hu, Miao, Zhang, Li, Hou, Nie, Liu: "Wnt/β-catenin links oxidative stress to podocyte injury and proteinuria." in: Kidney international, Vol. 95, Issue 4, pp. 830-845, (2020) (PubMed).

Eng, Kaverina, Schneider, Freedman, Gross, Miner, Pippin, Shankland: "Detection of renin lineage cell transdifferentiation to podocytes in the kidney glomerulus with dual lineage tracing." in: Kidney international, Vol. 93, Issue 5, pp. 1240-1246, (2018) (PubMed).

de Groot, Damen, Kosse, Alsady, Doty, Baumgarten, Sheehan, van der Vlag, Korstanje, Deen: "Lithium reduces blood glucose levels, but aggravates albuminuria in BTBR-ob/ob mice." in: PLoS ONE, Vol. 12, Issue 12, pp. e0189485, (2018) (PubMed).

Beckerman, Bi-Karchin, Park, Qiu, Dummer, Soomro, Boustany-Kari, Pullen, Miner, Hu, Rohacs, Inoue, Ishibe, Saleem, Palmer, Cuervo, Kopp, Susztak: "Transgenic expression of human APOL1 risk variants in podocytes induces kidney disease in mice." in: Nature medicine, Vol. 23, Issue 4, pp. 429-438, (2017) (PubMed).

Awad, You, Gao, Gvritishvili, Cooper, Tombran-Tink: "Delayed Treatment with a Small Pigment Epithelium Derived Factor (PEDF) Peptide Prevents the Progression of Diabetic Renal Injury." in: PLoS ONE, Vol. 10, Issue 7, pp. e0133777, (2016) (PubMed).

Badal, Wang, Long, Corcoran, Chang, Truong, Kanwar, Overbeek, Danesh: "miR-93 regulates Msk2-mediated chromatin remodelling in diabetic nephropathy." in: Nature communications, Vol. 7, pp. 12076, (2016) (PubMed).

Aggarwal, Veron, Thomas, Siegel, Moeckel, Kashgarian, Tufro: "Semaphorin3a promotes advanced diabetic nephropathy." in: Diabetes, Vol. 64, Issue 5, pp. 1743-59, (2015) (PubMed).

Veron, Villegas, Aggarwal, Bertuccio, Jimenez, Velazquez, Reidy, Abrahamson, Moeckel, Kashgarian, Tufro: "Acute podocyte vascular endothelial growth factor (VEGF-A) knockdown disrupts alphaVbeta3 integrin signaling in the glomerulus." in: PLoS ONE, Vol. 7, Issue 7, pp. e40589, (2013) (PubMed).

Veron, Bertuccio, Marlier, Reidy, Garcia, Jimenez, Velazquez, Kashgarian, Moeckel, Tufro: "Podocyte vascular endothelial growth factor (Vegf₁₆₄) overexpression causes severe nodular glomerulosclerosis in a mouse model of type 1 diabetes." in: Diabetologia, Vol. 54, Issue 5, pp. 1227-41, (2011) (PubMed).

Target Details for Nephrin

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Alternative Name: Nephrin (NPHS1 Products)

Synonyms: NPHS1 antibody, nphs1l antibody, nphs1 antibody, CNF antibody, NPHN antibody, nephrin antibody, NephrinB antibody, NPHS1, nephrin antibody, nephrosis 1, congenital, Finnish type (nephrin) antibody, NPHS1 nephrin S homeolog antibody, nephrin antibody, NPHS1 nephrin antibody, nephrosis 1, nephrin antibody, NPHS1 antibody, nphs1 antibody, nphs1.S antibody, LOC5579127 antibody, Nphs1 antibody

Pathways: Regulation of Actin Filament Polymerization, Skeletal Muscle Fiber Development