SLC25A14 antibody

Catalog No. ABIN286000

Product Details anti-SLC25A14 Antibody

Target: SLC25A14 (Solute Carrier Family 25 (Mitochondrial Carrier, Brain), Member 14 (SLC25A14))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC25A14 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Cross-Reactivity (Details): UCP5 may be alternatively spliced to produced at least 3 transcripts: UCP5L has a unique 22-aa unique N-terminal sequence, UCP5S is missing 3 aa (23-25 aa) and UCP5S has 31 aa insertion between TM II-IV. The UCP5 sequence is conserved in all 3 isoforms in rat and mouse UCP5. It is 94 % conserved in human UCP5/BMCP1. UCP5 has no appreciable homology with UCP1-4.

Purification: Affinity chromatography purified

Immunogen: UCP5 antibody was raised in rabbit using a 16 amino acid peptide from rat UCP5 as the immunogen.

Isotype: IgG

Application Details

Application Notes: ELISA: 1:10,000-1:100,000, WB: 1-10 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

UCP5Blocking Peptide, catalog no. 30R-AU004, is also available for use as a blocking control in assays to test for specificity of thisUCP5 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in PBS to a concentration of 1 mg/mL. Do not dilute further until ready to use.

Concentration: Lot specific

Buffer: Affinity purified IgG lyophilized from PBS with 0.1 % BSA, and 0.05 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for SLC25A14

Target: SLC25A14 (Solute Carrier Family 25 (Mitochondrial Carrier, Brain), Member 14 (SLC25A14))

Alternative Name: UCP5 (SLC25A14 Products)

Synonyms: ATPUMP5 antibody, DIC1 antibody, DICARBOXYLATE CARRIER 1 antibody, F14M13.10 antibody, F14M13_10 antibody, PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 5 antibody, uncoupling protein 5 antibody, zgc:55596 antibody, BMCP1 antibody, UCP5 antibody, BMCP-1 antibody, Ucp5 antibody, UCP5L antibody, UCP5S antibody, Bmcp1 antibody, solute carrier family 25 member 14 antibody, uncoupling protein 5 antibody, solute carrier family 25 (mitochondrial carrier, brain), member 14 antibody, SLC25A14 antibody, UCP5 antibody, slc25a14 antibody, Slc25a14 antibody

Background: Uncoupling proteins (UCP1-5) are a family of mitochondria transport proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. UCP3 is predicted to contain 6 transmembrane domains. UCP5 or Brain Mitochondrial Carrier Protein 1 or BMCP1 (human 322 aa, chromosome Xq24, ~35% identity with UCP1-3) is mainly expressed in the brain and testis.

Pathways: Proton Transport