GABARAPL1 antibody (C-Term)

Catalog No. ABIN2868936

Product Details anti-GABARAPL1 Antibody

Target: GABARAPL1 (GABA(A) Receptor-Associated Protein Like 1 (GABARAPL1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABARAPL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~14-16 kDa. Other observed bands at ~32 kDa.

Cross-Reactivity: Human, Rat

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide from the C-terminal of human GABARAPL1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN2868936 was sufficient for detection of GABARAPL1 on rat liver lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for GABARAPL1

Target: GABARAPL1 (GABA(A) Receptor-Associated Protein Like 1 (GABARAPL1))

Alternative Name: GABARAPL1 (GABARAPL1 Products)

Background: GABARAPL1, one of the proteins belonging to the GABARAP (GABA(A) receptor-associated protein) family, is highly expressed in the central nervous system and implicated in processes such as receptor and vesicle transport as well as autophagy. The proteins that make up the GABARAP family demonstrate conservation of their amino acid sequences and protein structures. In humans, GABARAPL1 shares 86 % identity with GABARAP and 61 % with GABARAPL2 (GATE-16) (1). GABARAPL1, or GABA(A) Receptor-Associated Protein Like 1, is a ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (2).

Gene ID: 23710

NCBI Accession: NP_113600

UniProt: Q9H0R8

Pathways: Autophagy