Collagen IV antibody

Catalog No. ABIN289410

Product Details anti-Collagen IV Antibody

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Characteristics: Affinity purified Rabbit polyclonal Collagen Type IV antibody
Bioactivity: Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues

Purification: Affinity chromatography purified

Sterility: Filtered

Immunogen: Collagen type IV antibody was raised in rabbit using collagen type IV from human and bovine placenta as the immunogen.

Application Details

Application Notes: ELISA: 1:5,000-1:20,000, IHC: 1:50-1:200, WB: 1:500-1:5,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Sterile filtered liquid in 0.125 M Na5 5 7, pH 8.0, with 0.075 NaCl, 0.005 EDTA, and 0.01 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

References for anti-Collagen IV antibody (ABIN289410)

Tane, Dhar, Roy, Pinheiro, Ohira, Roy: "Effect of excess synthesis of extracellular matrix components by trabecular meshwork cells: possible consequence on aqueous outflow." in: Experimental eye research, Vol. 84, Issue 5, pp. 832-42, (2007) (PubMed).

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen Type IV (COL4 Products)

Synonyms: collagen, type IV antibody, Let-2 antibody

Background: Collagen is a group of naturally occurring proteins found, in nature, exclusively in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content. Type-IV collagen is a type of collagen found primarily in the basal lamina. The C-terminus domain is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, type-IV lacks the regular glycine in every third residue necessary for the tight, collagen helix.