Macrophage Surface Antigen antibody

Catalog No. ABIN289946

Product Details

Target: Macrophage Surface Antigen

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Purification: The splenic lymphocytes from BALB/c mouse, immunized with human alveolarmacrophages, were fused to myeloma NS-1 cells. The screening of thehybridoma cells was performed on cryostat sections of human lung. The cellline (AM-3K) with positive reaction was grown in ascitic fluid of BALB/cmouse, from which the antibody was purified by Protein G affinity chromatography.

Immunogen: human alveolar macrophages

Clone: AM-3K

Isotype: IgG1

Application Details

Application Notes: Working dilution for immunohistochemistry: 10ug/ml, on frozen sections and paraffin sections. Antigen retrieval (microwave 10min, 0.01M citrate buffer, pH2.0) recommended.

Restrictions: For Research Use only

Handling

Concentration: 0.25 mg/ml

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Preservative: ProClin

Storage: -20 °C

Target Details

Target: Macrophage Surface Antigen

Background: This anti-macrophage monoclonal antibody, AM-3K, was produced by using human alveolarmacrophages as immunogen. AM-3K reacts intensely with most of macrophages in lymphoreticular organsand in many other organs and tissues. AM-3K also reacts with the macrophages in many pathologicalconditions. However, this antibody does not react with dendritic cell population, such as epidermal Langerhans cells, interdigitating cells in the paracortex of lymph nodes, nor follicular dendritic cells.Lymphocytes, granulocytes and freshly isolated blood monocytes are also negative. Reaction products for AM-3K were found on the cytoplasmic membrane of macrophages by immunoelectron microscopy. In both cryostat sections and formalin-fixed paraffin sections, this antibody recognizes the antigenpresenting on the cell surface membrane of tissue macrophages, but not monocytes or dendritic cells. The molecular weights of the antigen recognized by AM-3K are 120 and 70 kDa.