SCN5A antibody (AA 1548-1558)

Catalog No. ABIN290512

Product Details anti-SCN5A Antibody

Target: SCN5A (Sodium Channel, Voltage-Gated, Type V, alpha Subunit (SCN5A))

Binding Specificity: AA 1548-1558

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SCN5A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SCN5A / Nav1.5. This antibody is expected to recognise all reported isoforms (NP_932173.1, NP_000326.2, NP_001092874.1, NP_001092875.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Pig, Opossum (91%) Turkey, Chicken, Pufferfish, Slime mold (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-ETDDQSPEKIN from an internal region of human SCN5A / Nav1.5 (NP_932173.1, NP_000326.2, NP_001092874.1, NP_001092875.1, NP_001153632.1, NP_001153633.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Pig, Opossum (91%), Turkey, Chicken, Pufferfish, Slime mold (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 10 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SCN5A

Target: SCN5A (Sodium Channel, Voltage-Gated, Type V, alpha Subunit (SCN5A))

Alternative Name: SCN5A / Nav1.5 (SCN5A Products)

Synonyms: Nav1.5 antibody, Nav1.5c antibody, SkM1 antibody, SkM2 antibody, mH1 antibody, CDCD2 antibody, CMD1E antibody, CMPD2 antibody, HB1 antibody, HB2 antibody, HBBD antibody, HH1 antibody, ICCD antibody, IVF antibody, LQT3 antibody, PFHB1 antibody, SSS1 antibody, VF1 antibody, SCAL antibody, sodium voltage-gated channel alpha subunit 5 antibody, sodium channel, voltage-gated, type V, alpha antibody, SCN5A antibody, Scn5a antibody

Background: Name/Gene ID: SCN5A
Subfamily: Sodium channel - voltage-gated
Family: Ion Channel

Synonyms: SCN5A, CDCD2, CMPD2, HB1, HB2, HH1, HBBD, Nav1.5, IVF, RSKM2X, SCAL, Scn2x, LQT3, SSS1, MH1, VF1, PFHB1, RATRSKM2X, SkM2, CMD1E, ICCD, RH1, RSkM2

Gene ID: 6331

NCBI Accession: NP_932173, NP_000326, NP_001092874, NP_001092875, NP_001153632, NP_001153633

UniProt: Q14524