Nemo-Like Kinase antibody (AA 278-527)

Catalog No. ABIN3017553

Product Details anti-Nemo-Like Kinase Antibody

Target: Nemo-Like Kinase (NLK)

Binding Specificity: AA 278-527

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nemo-Like Kinase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: LKICDFGLAR VEELDESRHM TQEVVTQYYR APEILMGSRH YSNAIDIWSV GCIFAELLGR RILFQAQSPI QQLDLITDLL GTPSLEAMRT ACEGAKAHIL RGPHKQPSLP VLYTLSSQAT HEAVHLLCRM LVFDPSKRIS AKDALAHPYL DEGRLRYHTC MCKCCFSTST GRVYTSDFEP VTNPKFDDTF EKNLSSVRQV KEIIHQFILE QQKGNRVPLC INPQSAAFKS FISSTVAQPS EMPPSPLVWE

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 278-527 of human NLK (NP_057315.3).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Nemo-Like Kinase

Target: Nemo-Like Kinase (NLK)

Alternative Name: NLK (NLK Products)

Synonyms: Amf antibody, Gpi1 antibody, AI194375 antibody, nlk antibody, nlkb antibody, zgc:111875 antibody, Xnlk antibody, nlk1 antibody, nlk2 antibody, NLK antibody, nemo-like antibody, RGD1561602 antibody, glucose-6-phosphate isomerase antibody, nemo like kinase antibody, nemo-like kinase, type 1 antibody, nemo-like kinase L homeolog antibody, Nemo-Like kinase antibody, nemo-like kinase antibody, Gpi antibody, NLK antibody, Nlk antibody, nlk1 antibody, nlk.L antibody, nlk antibody, nemo-like antibody

Background: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members. Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner.,NLK,Epigenetics & Nuclear Signaling,Signal Transduction,Kinase,Serine/threonine kinases,Cell Biology & Developmental Biology,Cell Cycle,Wnt/β-Catenin Signaling Pathway,Stem Cells,NLK

Molecular Weight: 58 kDa

Gene ID: 51701

UniProt: Q9UBE8

Pathways: Ubiquitin Proteasome Pathway