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GFP antibody (Green Fluorescent Protein) Primary Antibody

GFP Reactivity: Aequorea victoria IF, WB Host: Mouse Monoclonal unconjugated
Pubmed (3)
Catalog No. ABIN3020573
$435.60
Plus shipping costs $45.00
100 μL ABIN3020573
100 μL ABIN3020573
local_shipping Shipping to: United States
Delivery in 10 to 11 Business Days
  • Target
    GFP
    Reactivity
    Aequorea victoria
    • 189
    • 7
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Host
    Mouse
    • 77
    • 75
    • 32
    • 16
    • 12
    • 7
    • 3
    • 1
    Clonality
    Monoclonal
    • 130
    • 89
    Conjugate
    This GFP antibody is un-conjugated
    • 121
    • 11
    • 10
    • 10
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Application
    Immunofluorescence (IF), Western Blotting (WB)
    • 156
    • 93
    • 49
    • 46
    • 37
    • 36
    • 32
    • 22
    • 14
    • 12
    • 10
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Purification
    Affinity purification
    Immunogen
    Fulllength recombinant GFP
    Isotype
    IgG
  • Application Notes
    WB: 1:2000-1:3000, IF: 1:50-1:100
    Restrictions
    For Research Use only
  • Buffer
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid freeze / thaw cycles
    Storage
    -20 °C / -80 °C
  • Xu, Liu, Zha, Fan, Zhang, Li, Xiao: "A pathogen-derived effector modulates host glucose metabolism by arginine GlcNAcylation of HIF-1α protein." in: PLoS pathogens, Vol. 14, Issue 8, pp. e1007259, 2019 (PubMed). Method employed by authors: Western Blotting (WB)

    Yang, Yang, Sun, Chen, Xu, Lai, Li, Wang, Bhattarai, Xiao, Sun, Zhu, Ma, Adhikari, Sun, Hao, Zhang, Huang, Huang, Jiang, Zhao, Wang, Sun, Yang: "5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m5C reader." in: Cell research, Vol. 27, Issue 5, pp. 606-625, 2018 (PubMed). Method employed by authors: Immunofluorescence (IF)

    Zhang, Lu, Liu, Cui, Wang: "Primate-specific miR-603 is implicated in the risk and pathogenesis of Alzheimer's disease." in: Aging, Vol. 8, Issue 2, pp. 272-90, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

  • Target
    GFP
    Alternative Name
    GFP
    Synonyms
    green fluorescent protein, gfp
    Background
    Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1, PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em).  
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