GFP antibody (Green Fluorescent Protein) Primary Antibody
This GFP antibody is un-conjugated
Immunofluorescence (IF), Western Blotting (WB)
- Affinity purification
- Fulllength recombinant GFP
anti-Green Fluorescent Protein (GFP) antibody (Abberior STAR 635P) Primary Antibody
GFP Reactivity: Aequorea victoria FACS, ICC, IF Host: Camelid (Camelidae) Monoclonal 1H1-1B2 Abberior STAR 635P single-domain Antibody (sdAb)
- Application Notes
- WB: 1:2000-1:3000, IF: 1:50-1:100
- For Research Use only
- PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid freeze / thaw cycles
- -20 °C / -80 °C
Xu, Liu, Zha, Fan, Zhang, Li, Xiao: "A pathogen-derived effector modulates host glucose metabolism by arginine GlcNAcylation of HIF-1α protein." in: PLoS pathogens, Vol. 14, Issue 8, pp. e1007259, 2019 (PubMed).
Method employed by authors: Western Blotting (WB)
Yang, Yang, Sun, Chen, Xu, Lai, Li, Wang, Bhattarai, Xiao, Sun, Zhu, Ma, Adhikari, Sun, Hao, Zhang, Huang, Huang, Jiang, Zhao, Wang, Sun, Yang: "5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m5C reader." in: Cell research, Vol. 27, Issue 5, pp. 606-625, 2018 (PubMed). Method employed by authors: Immunofluorescence (IF)
Zhang, Lu, Liu, Cui, Wang: "Primate-specific miR-603 is implicated in the risk and pathogenesis of Alzheimer's disease." in: Aging, Vol. 8, Issue 2, pp. 272-90, 2016 (PubMed). Method employed by authors: Western Blotting (WB)
- Xu, Liu, Zha, Fan, Zhang, Li, Xiao: "A pathogen-derived effector modulates host glucose metabolism by arginine GlcNAcylation of HIF-1α protein." in: PLoS pathogens, Vol. 14, Issue 8, pp. e1007259, 2019 (PubMed). Method employed by authors: Western Blotting (WB)
- Alternative Name
- green fluorescent protein, gfp
- Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1, PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em).