Goat anti-Rabbit IgG Antibody (HRP)

Details for Product No. ABIN3020597
Antigen
Reactivity
Rabbit
222
198
165
143
124
76
75
57
53
53
44
41
40
35
23
14
13
7
7
7
3
2
Host
Goat
576
499
185
44
36
32
7
7
1
Conjugate
HRP
181
161
138
131
129
127
46
35
32
29
28
24
16
11
9
9
9
9
7
6
5
5
5
5
5
5
5
5
5
4
4
4
2
2
2
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen Rabbit IgG
Isotype IgG
Characteristics Goat Anti-Rabbit IgG antibody is conjugated to horseradish peroxidase.
Purification Affinity purification
Antigen
Application Notes Recommended dilutions: WB: 1:2000-1:10000, IHC: 1:100-1:1000, ICC: 1:100-1:1000
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Product cited in: Xu, Huang, Liu, Chen, Chen, Yang, Zhao, Li, Duan, Liu, Yang: "Survivin regulated by autophagy mediates hyperglycemia-induced vascular endothelial cell dysfunction." in: Experimental cell research, Vol. 364, Issue 2, pp. 152-159, 2019 (PubMed). Method employed by authors: Western Blotting (WB)

Yang, Li, Wu, Shen, Zeng, Xiong, Wei, Chen, Liu, Huo, Li, Xue, Yao, Zhang, Li, Chen, Luo: "Comprehensive integrative analyses identify GLT8D1 and CSNK2B as schizophrenia risk genes." in: Nature communications, Vol. 9, Issue 1, pp. 838, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

Zhu, Shan, Chen, Cai, Cui, Yao, Wang, Shi, Tian, Lou, Xie, Wen: "Allosteric auto-inhibition and activation of the Nedd4 family E3 ligase Itch." in: EMBO reports, Vol. 18, Issue 9, pp. 1618-1630, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

Cheng, Geng, Dai, Zheng, Fu, Qiao, Cai, Wang, Yang: "Repression of let-7a cluster prevents adhesion of colorectal cancer cells by enforcing a mesenchymal phenotype in presence of liver inflammation." in: Cell death & disease, Vol. 9, Issue 5, pp. 489, 2018 (PubMed). Method employed by authors: Immunohistochemistry (IHC)

Nie, Huang, Deng, Bao, Liu, Feng, Wang, Chen, Qi, Pan: "The purine receptor P2X7R regulates the release of pro-inflammatory cytokines in human craniopharyngioma." in: Endocrine-related cancer, Vol. 24, Issue 6, pp. 287-296, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

Qiang, Jun-Jie, Hai, Hong, Bing-Xi, Lei, Wei, Ya-Wei, Huang, Song-Tao, Yun-Tao: "TPD52L2 impacts proliferation, invasiveness and apoptosis of glioblastoma cells via modulation of wnt/β-catenin/snail signaling." in: Carcinogenesis, Vol. 39, Issue 2, pp. 214-224, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

Sure, Chatterjee, Mishra, Sabharwal, Devireddy, Awasthi, Mohan, Koushika: "UNC-16/JIP3 and UNC-76/FEZ1 limit the density of mitochondria in C. elegans neurons by maintaining the balance of anterograde and retrograde mitochondrial transport." in: Scientific reports, Vol. 8, Issue 1, pp. 8938, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

Xiao, Zhang, Zhu, Liu, Lin, Wang, Xie: "CDK5-mediated tau accumulation triggers methamphetamine-induced neuronal apoptosis via endoplasmic reticulum-associated degradation pathway." in: Toxicology letters, Vol. 292, pp. 97-107, 2018 (PubMed). Method employed by authors: Immunofluorescence (IF), Immunohistochemistry (IHC)

Zhang, Di, Dong, Qu, Zhao, Duan, Hu, Liu, Zhou, Shi: "Epithelium-derived miR-204 inhibits corneal neovascularization." in: Experimental eye research, Vol. 167, pp. 122-127, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

Lv, Qiu, Wang, Liu, Xue, Yu, Song, Chen, Wang, Song: "A GTP-dependent Phosphoenolpyruvate Carboxykinase from Crassostrea gigas Involved in Immune Recognition." in: Developmental and comparative immunology, Vol. 77, pp. 318-329, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

Hou, Wang, He, Li, Xie, Zhao, Zhao: "Nrf2 inhibits NLRP3 inflammasome activation through regulating Trx1/TXNIP complex in cerebral ischemia reperfusion injury." in: Behavioural brain research, Vol. 336, pp. 32-39, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

He, Li, Wang, Hou, Li, Zhao: "Resveratrol alleviates cerebral ischemia/reperfusion injury in rats by inhibiting NLRP3 inflammasome activation through Sirt1-dependent autophagy induction." in: International immunopharmacology, Vol. 50, pp. 208-215, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

Zhang, Li, Zhang, Gao, Dai: "Astragaloside IV inhibits Angiotensin II-stimulated proliferation of rat vascular smooth muscle cells via the regulation of CDK2 activity." in: Life sciences, Vol. 200, pp. 105-109, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

Zhou, Du, Liu, Zhao, Yuan, Li, Chen, Lu, Cheng, Liu: "Polyacetylene glycoside attenuates ischemic kidney injury by co-inhibiting inflammation, mitochondria dysfunction and lipotoxicity." in: Life sciences, Vol. 204, pp. 55-64, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

Chen, Xing, Ma, Luo, Su, Li, Shi, He: "N-myc downstream-regulated gene 1 facilitates influenza A virus replication by suppressing canonical NF-κB signaling." in: Virus research, Vol. 252, pp. 22-28, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

Jiao, Zhang, Zhang, Yang, Wang, Zhao: "Efficient co-expression of multiple enzymes from a single promoter mediated by virus 2A sequence in the oleaginous yeast Rhodosporidium toruloides." in: FEMS yeast research, Vol. 18, Issue 8, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

Li, Lu, Bai, Xiao, Guo, Dong: "Ginsenoside Rg3 Suppresses Proliferation and Induces Apoptosis in Human Osteosarcoma." in: BioMed research international, Vol. 2018, pp. 4306579, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

Fu, Ke, Yu, Wang, Xiao, Gu, Lü: "Periodic Variation of AAK1 in an Aβ1-42-Induced Mouse Model of Alzheimer's Disease." in: Journal of molecular neuroscience : MN, Vol. 65, Issue 2, pp. 179-189, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

Li, Liu, Fan, Zhou, Song, Peng, Qin, Tao: "Validation and bioinformatic analysis of propofol-induced differentially expressed microRNAs in primary cultured neural stem cells." in: Gene, Vol. 664, pp. 90-100, 2018 (PubMed). Method employed by authors: Western Blotting (WB)

Zhang, Liu, Chen, Yang, Liu, Li, Wang, Li, Liu: "Microencapsulated Schwann cell transplantation inhibits P2X2/3 receptors overexpression in a sciatic nerve injury rat model with neuropathic pain." in: Neuroscience letters, Vol. 676, pp. 51-57, 2018 (PubMed). Method employed by authors: Western Blotting (WB)