Clathrin Heavy Chain (CLTC) antibody

Catalog No. ABIN302098

Product Details

Target: Clathrin Heavy Chain (CLTC)

Reactivity: Human, Mouse, Rat, Cow, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificity: The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa intracellular protein involved in receptor-mediated endocytosis.

Cross-Reactivity (Details): Human, Porcine, Mouse, Rat, Bovine, Other not determined

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Clone: BF-06

Isotype: IgM

Application Details

Application Notes: Flow cytometry: Recommended dilution: 2-5 μg/mL, positive control: human blood leukocytes. Intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References

Mac?rek, Zíková, Dráberová, Dráber: "Monoclonal antibody BF-06 against the heavy chain of clathrin." in: Folia biologica, Vol. 49, Issue 6, pp. 238-40, (2004) (PubMed).

Target Details

Target: Clathrin Heavy Chain (CLTC)

Alternative Name: Clathrin heavy chain (CLTC Products)

Background: Clathrin heavy chain,Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.,CHC, CLHC, MRD56

Gene ID: 1213

UniProt: Q00610