OTUD5 antibody (Internal Region)

Catalog No. ABIN302160

Product Details anti-OTUD5 Antibody

Target: OTUD5 (OTU Domain Containing 5 (OTUD5))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This OTUD5 antibody is un-conjugated

Application: ELISA

Purpose: DUBA

Sequence: RQVRGPSQPR KAS

Predicted Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Recent

Immunogen: Peptide with sequence C-RQVRGPSQPRKAS, from the internal region of the protein sequence according to NP_060072.1.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Preliminary experiments gave an approx 80 kDa band in lysates of cell lines Jurkat and MOLT4 after 0.2 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 60.6 kDa according to NP_060072.1. The 80 kDa band was successfully blocked by incubation with the immunizing peptide. Have any further splice variants/modified forms been reported?

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for OTUD5

Target: OTUD5 (OTU Domain Containing 5 (OTUD5))

Alternative Name: OTUD5 (OTUD5 Products)

Background: OTUD5, OTU domain containing 5, DKFZp761A052, DUBA, MGC104871, -

Gene ID: 55593

NCBI Accession: NP_060072