TTF1 antibody
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- Target See all TTF1 Antibodies
- TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))
- Reactivity
- Human, Rat, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TTF1 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
- Purification
- Protein G affinity chromatography
- Immunogen
- Recombinant protein was used as the immunogen for the TTF1 antibody.
- Clone
- HBNK2-1
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product TTF1 Primary Antibody
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- Application Notes
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Titering of the TTF1 antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH 6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the TTF1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))
- Alternative Name
- TTF1 (TTF1 Products)
- Synonyms
- TTF-1 antibody, TTF-I antibody, AV245725 antibody, RGD1565673 antibody, transcription termination factor 1 antibody, transcription termination factor, RNA polymerase I antibody, TTF1 antibody, Ttf1 antibody
- Background
- Thyroid transcription factor-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. TTF-1 reactivity is also seen in thyroid malignancies.
- Pathways
- Thyroid Hormone Synthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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