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TTF1 antibody

TTF1 Reactivity: Human, Rat, Mouse FACS, IHC (p), IF Host: Mouse Monoclonal HBNK2-1 unconjugated
Catalog No. ABIN3023953
  • Target See all TTF1 Antibodies
    TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))
    Reactivity
    Human, Rat, Mouse
    Host
    • 48
    • 8
    Mouse
    Clonality
    • 45
    • 11
    Monoclonal
    Conjugate
    • 27
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TTF1 antibody is un-conjugated
    Application
    • 33
    • 21
    • 15
    • 13
    • 10
    • 7
    • 6
    • 4
    • 3
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Purification
    Protein G affinity chromatography
    Immunogen
    Recombinant protein was used as the immunogen for the TTF1 antibody.
    Clone
    HBNK2-1
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product TTF1 Primary Antibody
  • Application Notes
    Titering of the TTF1 antibody may be required for optimal performance.

    1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    0.2 mg/mL
    Buffer
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the TTF1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))
    Alternative Name
    TTF1 (TTF1 Products)
    Synonyms
    TTF-1 antibody, TTF-I antibody, AV245725 antibody, RGD1565673 antibody, transcription termination factor 1 antibody, transcription termination factor, RNA polymerase I antibody, TTF1 antibody, Ttf1 antibody
    Background
    Thyroid transcription factor-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. TTF-1 reactivity is also seen in thyroid malignancies.
    Pathways
    Thyroid Hormone Synthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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