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Actin antibody

ACTA1 Reactivity: Human, Rat WB, IHC (p), IF Host: Mouse Monoclonal PMAC1-1 unconjugated
Catalog No. ABIN3024131
  • Target See all Actin (ACTA1) Antibodies
    Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))
    Reactivity
    • 96
    • 44
    • 43
    • 17
    • 12
    • 10
    • 9
    • 8
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat
    Host
    • 81
    • 31
    Mouse
    Clonality
    • 76
    • 34
    Monoclonal
    Conjugate
    • 63
    • 12
    • 12
    • 10
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Actin antibody is un-conjugated
    Application
    • 91
    • 57
    • 40
    • 28
    • 27
    • 25
    • 10
    • 9
    • 7
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Purification
    Protein G affinity chromatography
    Immunogen
    An amino acid sequence common among human muscle actins was used as the immunogen for the Muscle Actin antibody.
    Clone
    PMAC1-1
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product ACTA1 Primary Antibody
  • Application Notes
    Titering of the Muscle Actin antibody may be required for optimal performance.

    1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))
    Alternative Name
    Muscle Actin (ACTA1 Products)
    Synonyms
    ACTA antibody, ASMA antibody, CFTD antibody, CFTD1 antibody, CFTDM antibody, MPFD antibody, NEM1 antibody, NEM2 antibody, NEM3 antibody, acta antibody, asma antibody, cftd antibody, cftd1 antibody, cftdm antibody, mpfd antibody, nem1 antibody, AA959943 antibody, Acta-2 antibody, Acts antibody, Actsk-1 antibody, actc antibody, actc1 antibody, cfk antibody, hm:zeh0631 antibody, zeh0631 antibody, acta1 antibody, hm:zeh0293 antibody, zgc:112098 antibody, actin, alpha 1, skeletal muscle antibody, actin, alpha 1, skeletal muscle L homeolog antibody, actin, alpha 1b, skeletal muscle antibody, actin, muscle antibody, actin, alpha skeletal muscle antibody, actin, alpha 1a, skeletal muscle antibody, ACTA1 antibody, acta1.L antibody, acta1 antibody, Acta1 antibody, acta1b antibody, LOC100533357 antibody, LOC109479779 antibody, LOC109108280 antibody, acta1a antibody
    Background
    Actin participates in many important cellular processes, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. An actin protein's mass is roughly 42-  kDa and it is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. It can be present as either a free monomer called G-actin (globular) or as part of a linear polymer microfilament called F-actin (filamentous), both of which are essential for such important cellular functions as the mobility and contraction of cells during cell division. [Wiki]

    This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells.

    Pathways
    Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
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