CD31 antibody
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- Target See all CD31 (PECAM1) Antibodies
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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Reactivity
- Human, Rabbit, Cynomolgus
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD31 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- No Cross-Reactivity
- Rat (Rattus), Pig (Porcine)
- Purification
- Protein G affinity chromatography
- Immunogen
- A membrane preparation of a spleen from a patient with hairy cell leukemia was used as the immunogen for this CD31 antibody.
- Clone
- JC-70A
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product PECAM1 Primary Antibody
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- Application Notes
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The optimal dilution of the CD31 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the CD31 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes." in: Stem cells international, Vol. 2017, pp. 1709582, (2017) (PubMed).
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Adipose-Derived Stem Cells Cocultured with Chondrocytes Promote the Proliferation of Chondrocytes." in: Stem cells international, Vol. 2017, pp. 1709582, (2017) (PubMed).
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- Target
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
- Alternative Name
- CD31 (PECAM1 Products)
- Synonyms
- CD31 antibody, CD31/EndoCAM antibody, GPIIA' antibody, PECA1 antibody, PECAM-1 antibody, endoCAM antibody, C85791 antibody, Cd31 antibody, Pecam antibody, platelet/endothelial cell adhesion molecule 1 antibody, platelet and endothelial cell adhesion molecule 1 antibody, pecam1 antibody, PECAM1 antibody, Pecam1 antibody
- Background
- CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 mAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
- Pathways
- Regulation of Actin Filament Polymerization
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