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CDC20 antibody

CDC20 Reactivity: Human FACS, IF, IHC (p) Host: Mouse Monoclonal CDC20-1102 unconjugated
Catalog No. ABIN3024581
  • Target See all CDC20 Antibodies
    CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
    Reactivity
    • 57
    • 21
    • 17
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 44
    • 14
    Mouse
    Clonality
    • 43
    • 15
    Monoclonal
    Conjugate
    • 34
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CDC20 antibody is un-conjugated
    Application
    • 36
    • 23
    • 20
    • 13
    • 12
    • 10
    • 9
    • 4
    • 2
    • 2
    • 2
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    Protein G affinity chromatography
    Immunogen
    Recombinant human protein was used as the immunogen for the Cdc20 antibody.
    Clone
    CDC20-1102
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product CDC20 Primary Antibody
  • Application Notes
    Optimal dilution of the Cdc20 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    0.2 mg/mL
    Buffer
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the Cdc20 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
    Alternative Name
    Cdc20 (CDC20 Products)
    Synonyms
    X-FZY antibody, ba276h19.3 antibody, cdc20a antibody, fizzy1 antibody, p55cdc antibody, CDC20A antibody, bA276H19.3 antibody, p55CDC antibody, 2310042N09Rik antibody, C87100 antibody, cell division cycle 20 antibody, cell division cycle 20 L homeolog antibody, cell division cycle 20 homolog (S. cerevisiae) antibody, cdc20 antibody, CDC20 antibody, cdc20.L antibody, Cdc20 antibody
    Background
    Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
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