ATF6 antibody (N-Term)

Catalog No. ABIN3024785

Product Details anti-ATF6 Antibody

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G purified monoclonal antibody

Immunogen: A recombinant protein fragment from the N-terminal region of human ATF6 was used as the immunogen for this antibody.

Clone: AF6229

Isotype: IgG1 kappa

Application Details

Application Notes: ATF6 is a 670 amino acid protein that migrates in western blot between 70~90 kD due to glycosylation.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. ATF6 is seen in western blotting between 75~90KD.\. Western blot: 2-4 μg/mL (2),IHC (Paraffin): 5 μg/mL (1)

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US source) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for ATF6

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Alternative Name: ATF6 (ATF6 Products)

Synonyms: ATF-6-like antibody, GB16435 antibody, ATF6 antibody, Atf6 antibody, ATF6A antibody, 9130025P16Rik antibody, 9630036G24 antibody, AA789574 antibody, Atf6alpha antibody, ESTM49 antibody, activating transcription factor 6 antibody, uncharacterized LOC412432 antibody, activating transcription factor 6 S homeolog antibody, cyclic AMP-dependent transcription factor ATF-6 alpha antibody, ATF6 antibody, LOC412432 antibody, atf6.S antibody, LOC100122162 antibody, Atf6 antibody

Background: ER stress can cause the accumulation of unfolded proteins and lead to the activation of the UPR (unfolded proetin response). Cells have developed an intracellular signaling pathway to deal with this stress, and Activating Transcription Factor 6 (ATF6) is a major transducer of the UPR. Upon activation, ATF6 seperates from GPR78 and is cleaved. The ~50 kD cytosolic portion of ATF6 translocates to the nucleus, transmitting the ER stress signal. In the nucleus, ATF6 activates the transcription of UPR-responsive genes by biniding to their ERSE (ER stress response element) region.

Gene ID: 22926

Pathways: ER-Nucleus Signaling, Unfolded Protein Response