Recombinant Nkx2-2 antibody

Catalog No. ABIN3025345

Product Details anti-Nkx2-2 Antibody

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein A affinity chromatography

Immunogen: A human partial recombinant protein was used as the immunogen for this rabbit monoclonal NKX2.2 antibody.

Clone: RMNK2-1

Isotype: IgG kappa

Application Details

Application Notes: Titering of the NKX2.2 antibody may be required for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the NKX2.2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2.2 (Nkx2-2 Products)

Synonyms: XeNK-2 antibody, Xnkx2.2 antibody, nkx2-2-A antibody, nkx2.2 antibody, NKX-2.2 antibody, NKX2.2 antibody, NKX2B antibody, Nkx-2.2 antibody, Nkx2.2 antibody, tinman antibody, id:ibd5090 antibody, nk2.2 antibody, nkx2-2 antibody, wu:fc83b01 antibody, zgc:136536 antibody, NK2 homeobox 2 S homeolog antibody, NK2 homeobox 2 antibody, NK2 homeobox 2a antibody, nkx2-2.S antibody, NKX2-2 antibody, Nkx2-2 antibody, nkx2.2a antibody

Background: NKX2.2 acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity). [UniProt]

Gene ID: 4821

Pathways: Dopaminergic Neurogenesis