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CD28 antibody

CD28 Reactivity: Human, Mouse, Cow, Pig, Sheep FACS, IF, Func Host: Mouse Monoclonal 204-12 unconjugated

Catalog No. ABIN3025652

Datasheet as PDF

Target See all CD28 Antibodies

CD28
Reactivity
173

128

58

38

21

20

12

12

7

7

6

5

1

1

1

1
Human, Mouse, Cow, Pig, Sheep
Host
126

79

33

16

8

3

2

1
Mouse
Compatible Secondaries
Clonality
188

74

3
Monoclonal
Conjugate
88

30

29

21

14

6

5

5

5

4

4

4

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This CD28 antibody is un-conjugated
Application
199

94

47

47

38

31

27

26

19

17

13

7

7

6

4

4

4

3

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Purification

Protein G affinity chromatography

Immunogen

Stimulated human leukocytes were used as the immunogen for the CD28 antibody.

Clone

204-12

Isotype

IgG2a kappa

Top Product

Discover our top product CD28 Primary Antibody
anti-CD28 (CD28) (AA 1-120) antibody
CD28 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-CD28 (CD28) antibody
CD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) antibody (FITC)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 FITC

anti-CD28 (CD28) (AA 19-152) antibody
CD28 Reactivity: Human FACS, ELISA Host: Rabbit Monoclonal DM61 unconjugated Recombinant Antibody

anti-CD28 (CD28) antibody
CD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) antibody
CD28 Reactivity: Rat FACS, IP Host: Mouse Monoclonal JJ319 unconjugated

anti-CD28 (CD28) (C-Term) antibody
CD28 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-CD28 (CD28) antibody (PE)
CD28 Reactivity: Rat FACS Host: Mouse Monoclonal JJ319 PE

anti-CD28 (CD28) (Internal Region) antibody
Verified CD28 Reactivity: Human WB, ELISA, IHC Host: Goat Polyclonal unconjugated

anti-CD28 (CD28) antibody (APC)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 APC

Application Notes

Optimal dilution of the CD28 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Functional Activity (Order SAF formulation)

Restrictions

For Research Use only
Concentration

0.2 mg/mL

Buffer

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store the CD28 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Target

CD28

Alternative Name

CD28 (CD28 Products)

Synonyms

Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody

Background

Recognizes a glycoprotein of 44-88  kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Pathways

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response