Fascin antibody

Catalog No. ABIN3025905

Product Details anti-Fascin Antibody

Target: Fascin (FSCN1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fascin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Full length recombinant human protein was used as the immunogen for the Fascin antibody.

Clone: FSCN1-417

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal dilution of the Fascin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Fascin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Fascin

Target: Fascin (FSCN1)

Alternative Name: Fascin (FSCN1 Products)

Synonyms: CG15331 antibody, CG1536 antibody, CG32858 antibody, Dmel\\CG32858 antibody, SN antibody, Sn antibody, fs(1)A1057 antibody, fs(1)K1421 antibody, fs(1)K418 antibody, fs(1)K473 antibody, fs(1)K743 antibody, fs(1)M45 antibody, p55 antibody, snl antibody, MGC75811 antibody, fscn1 antibody, sb:cb589 antibody, zgc:153632 antibody, AI663989 antibody, Fan1 antibody, fascin-1 antibody, Fascin antibody, fscn antibody, FAN1 antibody, HSN antibody, SNL antibody, fascin antibody, singed antibody, fascin actin-bundling protein 1 antibody, fascin actin-bundling protein 1a antibody, fascin actin-bundling protein 1 L homeolog antibody, sn antibody, fscn1 antibody, fscn1a antibody, Fscn1 antibody, fscn1.L antibody, FSCN1 antibody

Background: Recognizes a protein of 55  kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.