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ICAM-3/CD50 antibody

ICAM3 Reactivity: Human FACS, WB, IF, IHC (p) Host: Mouse Monoclonal ICAM3-1019 unconjugated
Catalog No. ABIN3026061
  • Target See all ICAM-3/CD50 (ICAM3) Antibodies
    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
    Reactivity
    • 113
    • 3
    • 2
    • 2
    Human
    Host
    • 68
    • 44
    • 1
    Mouse
    Clonality
    • 73
    • 40
    Monoclonal
    Conjugate
    • 62
    • 10
    • 6
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ICAM-3/CD50 antibody is un-conjugated
    Application
    • 62
    • 50
    • 47
    • 34
    • 26
    • 25
    • 23
    • 13
    • 7
    • 7
    • 7
    • 6
    • 6
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    Protein G affinity chromatography
    Immunogen
    Recombinant human protein was used as the immunogen for the ICAM3 antibody.
    Clone
    ICAM3-1019
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product ICAM3 Primary Antibody
  • Application Notes
    Optimal dilution of the ICAM3 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    0.2 mg/mL
    Buffer
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the ICAM3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
    Alternative Name
    ICAM3 (ICAM3 Products)
    Synonyms
    CD50 antibody, CDW50 antibody, ICAM-R antibody, ICAM-3 antibody, ICAM3 antibody, intercellular adhesion molecule 3 antibody, ICAM3 antibody, Icam3 antibody
    Background
    Recognizes an N-glycosylated glycoprotein of 120  kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb is excellent for staining of formalin/paraffin tissues.
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