TAG-72 antibody

Catalog No. ABIN3026069

Product Details anti-TAG-72 Antibody

Target: TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))

Reactivity: Human, Rat, Cow, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This TAG-72 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Purification: Protein G affinity chromatography

Immunogen: Purified TAG-72 protein was used as the immunogen for this TAG-72 antibody.

Clone: CC49

Isotype: IgG1

Application Details

Application Notes: The optimal dilution of the TAG-72 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the TAG-72 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for TAG-72

Target: TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))

Alternative Name: TAG-72 (TAG-72 Products)

Synonyms: mRNA cap guanine-N7 methyltransferase antibody, tag-72 antibody

Background: Recognizes an oncofetal antigen of 220  kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This mAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.