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Mouse anti-Human kappa Light Chain Antibody

FACS, WB, IF, IHC (p) Monoclonal L1C1 IgG1 kappa unconjugated
Catalog No. ABIN3026391
  • Target See all kappa Light Chain products
    kappa Light Chain
    Reactivity
    • 34
    • 11
    Human
    Host
    • 17
    • 11
    • 9
    • 4
    • 3
    • 1
    Mouse
    Clonality
    • 26
    • 19
    Monoclonal
    Application
    • 29
    • 26
    • 18
    • 15
    • 14
    • 10
    • 9
    • 8
    • 8
    • 6
    • 5
    Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    No Cross-Reactivity
    Rat (Rattus)
    Characteristics
    This antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
    Purification
    Protein G affinity chromatography
    Immunogen
    Human B-Lymphoma Cells were used as the immunogen for this Kappa light chain antibody.
    Clone
    L1C1
    Isotype
    IgG1 kappa
  • Application Notes
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-2.0 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    0.2 mg/mL
    Buffer
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the Kappa light chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    kappa Light Chain
    Abstract
    kappa Light Chain Products
    Background
    This antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
    Gene ID
    3514
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