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AQP3 antibody (C-Term)

AQP3 Reactivity: Human, Rat, Mouse WB, IHC (p), ICC, IHC (fro), FACS Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3030005
  • Target See all AQP3 Antibodies
    AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))
    Binding Specificity
    • 18
    • 15
    • 9
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 46
    • 42
    • 37
    • 4
    • 4
    • 1
    • 1
    • 1
    Human, Rat, Mouse
    Host
    • 60
    Rabbit
    Clonality
    • 60
    Polyclonal
    Conjugate
    • 24
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This AQP3 antibody is un-conjugated
    Application
    • 52
    • 29
    • 21
    • 21
    • 16
    • 13
    • 13
    • 6
    • 5
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
    Purification
    Antigen affinity
    Immunogen
    An amino acid sequence from the C-terminus of human Aquaporin 3 (EENVKLAHVKHKEQI) was used as the immunogen for this AQP3 antibody.
    Isotype
    IgG
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  • Application Notes
    The stated application concentrations are suggested starting amounts. Titration of the AQP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells
    Restrictions
    For Research Use only
  • Buffer
    0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
    Storage
    -20 °C
    Storage Comment
    After reconstitution, the AQP3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
  • Target
    AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))
    Alternative Name
    AQP3 Aquaporin 3 (AQP3 Products)
    Synonyms
    aqp3 antibody, wu:fa95h06 antibody, wu:fi13e05 antibody, zgc:55525 antibody, Tb06.28P18.240 antibody, AQP-2 antibody, AQP-3 antibody, GIL antibody, SAQP3 antibody, aquaporin 3a antibody, aquaporin 3 (Gill blood group) antibody, aquaporin 3 antibody, aqp3a antibody, AQP3 antibody, Tb927.6.1520 antibody, MCYG_07750 antibody, Aqp3 antibody
    Background
    Aquaporin 3 is the third member of the major intrinsic protein family. The protein is localized at the basal lateral membranes of collecting duct cells in the kidney. AQP3 appears to exist as a single copy and to comprise 6 exons distributed over 7 kb. The gene is mapped to 9p21-p12 by fluorescence in situ hybridization. The protein works as water-channel and facilitates the transport of nonionic small solutes such as urea and glycerol, albeit to a smaller degree. The results suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. By Western blot analysis and immunofluorescence microscopy of epidermis, Sougrat et al.(2002) showed strong expression of an approximately 33-kD glycosylated AQP3 protein in keratinocyte plasma membranes, one layer below the unstained stratum corneum(SC). It provides a short circuit for water, or water-clamp, between the base of the epidermis and the SC in order to maintain a constant H2O content and to prevent the formation of a continuous water gradient across the epidermis below the SC.
    UniProt
    Q92482
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