DAXX antibody (AA 325-341)

Catalog No. ABIN3030705

Product Details anti-DAXX Antibody

Target: DAXX (Death-Domain Associated Protein (DAXX))

Binding Specificity: AA 325-341

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAXX antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogen: Amino acids 325-341 (QERRHLDLIYNFGCHLT) were used as the immunogen for this Daxx antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Daxx antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Daxx antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for DAXX

Target: DAXX (Death-Domain Associated Protein (DAXX))

Alternative Name: Daxx (DAXX Products)

Synonyms: BING2 antibody, DAP6 antibody, EAP1 antibody, LOC100226911 antibody, daxx antibody, si:dz179b20.7 antibody, wu:fd15c03 antibody, death domain associated protein antibody, Fas death domain-associated protein antibody, 28S ribosomal protein S29, mitochondrial antibody, death-domain associated protein antibody, death-associated protein 6 L homeolog antibody, DAXX antibody, Daxx antibody, LOC100226911 antibody, daxx.L antibody, daxx antibody

Background: Death-domain associated protein, also known as DAP6 (Death-associated protein 6) or BING2, was first discovered through its cytoplasmic interaction with the classical death receptor Fas. The gene encodes a 740-amino acid polypeptide containing a nuclear localization signal. Functional analyses byYang et al.(1997) demonstrated that Daxx binds to the Fas death domain and enhances Fas-mediated apoptosis. The authors suggested that DAXX and FADD define 2 distinct apoptotic pathways downstream of Fas. The gene is mapped to human chromosome 6p21.3 by somatic cell hybrid panels and fluorescence in situ hybridization, a region containing the HLA and putative autoimmune disease genes. MSP58 overexpression relieved DAXX-mediated transcriptional repression. Immunoprecipitation and Western blot analysis with DAXX mutants showed that the N terminus of the protein interacts with the C terminus of DMAP. Transient expression of DAXX or DMAP1 caused repression of glucocorticoid receptor-mediated transcription.

UniProt: Q9UER7

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway