EIF6 antibody (Middle Region)

Catalog No. ABIN3030895

Product Details anti-EIF6 Antibody

Target: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human Eukaryotic Translation Initiation Factor 6 (QHIRNSLPDTVQIRR) was used as the immunogen for this EIF6 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the EIF6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the EIF6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for EIF6

Target: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Alternative Name: EIF6 (EIF6 Products)

Synonyms: CAB antibody, EIF3A antibody, ITGB4BP antibody, b(2)gcn antibody, eIF-6 antibody, p27(BBP) antibody, p27BBP antibody, 1110004P11 antibody, AA408895 antibody, Itgb4bp antibody, imc-415 antibody, MGC56562 antibody, itgb4bp4 antibody, zgc:56562 antibody, wu:fc28a04 antibody, wu:ft88f05 antibody, itgb4bp antibody, eukaryotic translation initiation factor 6 antibody, Eukaryotic translation initiation factor 6 antibody, eukaryotic translation initiation factor 6 S homeolog antibody, translation initiation factor eIF6 antibody, EIF6 antibody, Eif6 antibody, eif6 antibody, eif-6 antibody, eif6.S antibody, tif6 antibody

Background: EIF6 (Eukaryotic Translation Initiation Factor 6), also called ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al.(1998) mapped the gene to 20q11.2. Ceci et al.(2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, it is bound to free 60S but not to 80S subunits. Furthermore, the protein interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al.(2008) demonstrated that the protein is required for efficient initiation of translation in vivo. Null mouse embryos were lethal at preimplantation. Heterozygous mice had 50 % reduction of EIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.

UniProt: P56537

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly