NOXA1 antibody (Middle Region)

Catalog No. ABIN3031937

Product Details anti-NOXA1 Antibody

Target: NOXA1 (NADPH Oxidase Activator 1 (NOXA1))

Binding Specificity: Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOXA1 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human NADPH oxidase activator 1 (RQVPRGEVFRPHRWHLKHLE) was used as the immunogen for this NOXA1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the NOXA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the NOXA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for NOXA1

Target: NOXA1 (NADPH Oxidase Activator 1 (NOXA1))

Alternative Name: NOXA1 (NOXA1 Products)

Synonyms: NY-CO-31 antibody, SDCCAG31 antibody, p51NOX antibody, NADPH oxidase activator 1 antibody, NOXA1 antibody, Noxa1 antibody

Background: NADPH oxidase activator 1, also called NOX Activator 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz(2007) mapped the gene to chromosome 9q34.3 based on an alignment of the protein sequence with the genomic sequence(build 36.1). Banfi et al.(2003) mapped the mouse gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al.(2003) showed that the human protein interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that NOXA1 bound GTP-bound RAC1, but not GDP-bound RAC1. It also bound p47-PHOX (NCF1) and p41-NOX (NOXO1), and trp436 within the SH3 domain of NOXA1 was required for these interactions. Human cell lines or COS-7 cells cotransfected with NOXA1/p51-NOX and p41-NOX and either gp91-PHOX(CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.

UniProt: Q86UR1