NOL3 antibody (AA 91-106)

Catalog No. ABIN3032070

Product Details anti-NOL3 Antibody

Target: NOL3 (Nucleolar Protein 3 (Apoptosis Repressor with CARD Domain) (NOL3))

Binding Specificity: AA 91-106

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOL3 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogen: Amino acids 91-106 (WDWQHVGPGYRDRSYD-human) were used as the immunogen for this ARC antibody (100% rat homology).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the ARC antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the ARC antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for NOL3

Target: NOL3 (Nucleolar Protein 3 (Apoptosis Repressor with CARD Domain) (NOL3))

Alternative Name: ARC Apoptosis Repressor with CARD (NOL3 Products)

Synonyms: ARC antibody, FCM antibody, MYP antibody, NOP antibody, NOP30 antibody, B430311C09Rik antibody, MYC antibody, Nop30 antibody, Arc antibody, nucleolar protein 3 antibody, nucleolar protein 3 (apoptosis repressor with CARD domain) antibody, NOL3 antibody, Nol3 antibody

Background: Apoptosis Repressor with CARD, also known as ARC, NOP30, NOL3, CARD2 and MYP, is a protein that in humans is encoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic sequence analysis, Stoss et al.(1999) determined that the NOL3 gene, which encodes NOP30 and MYP and which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates the two isoforms is located in exon 2. It is reported that expression of the Apoptosis Repressor with CARD/ARC cDNA encoding the smaller transcript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when coexpressed with CASP9. The protein also inhibited apoptosis induced by stimulation of CD95/FAS, tumor necrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits the enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC interacts with CASP2 and -8 through its N-terminal death effector domain but does not interact with CASP1, -3, or -9.

UniProt: O60936