The stated application concentrations are suggested starting amounts. Titration of the Nucleophosmin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage
-20 °C
Storage Comment
After reconstitution, the Nucleophosmin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
NPM1 or NPM (Nucleophosmin/Nucleoplasmin family member1), also known as Nucleolar phosphoprotein B23 or numatrin, is a protein that in humans is encoded by the NPM1 gene. Chan et al. (1989) found that NPM is a nucleolar phosphoprotein that is more abundant in tumor cells than in normal resting cells. Stimulation of the growth of normal cells, e.g., mitogen activation of B lymphocytes, was accompanied by an increase in Nucleophosmin protein level. They stated that the protein is likely involved in the assembly of ribosomal proteins into ribosomes. Electron microscopic study indicated that Nucleophosmin is concentrated in the granular region of the nucleolus, where ribosome assembly occurs.