OSBP antibody (Middle Region)

Catalog No. ABIN3032108

Product Details anti-OSBP Antibody

Target: OSBP (Oxysterol Binding Protein (OSBP))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OSBP antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human Oxysterol-Binding Protein (WQKSLQYERDQRIRL) was used as the immunogen for this OSBP antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the OSBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the OSBP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for OSBP

Target: OSBP (Oxysterol Binding Protein (OSBP))

Alternative Name: OSBP (OSBP Products)

Synonyms: CG6708 antibody, Dmel\\CG6708 antibody, OSBP-Dm antibody, osbp antibody, OSBP1 antibody, 1110018F06Rik antibody, AW559088 antibody, mKIAA4220 antibody, Oxysterol binding protein antibody, oxysterol binding protein antibody, Osbp antibody, OSBP antibody

Background: Oxysterol-Binding Protein is a protein that in humans is encoded by the OSBP gene. Im et al.(2005) reported the structure of the full-length yeast oxysterol-binding-protein Osh4, a member of the OSBP-related protein (ORP) family, at 1.5- to 1.9-angstrom resolution in complexes with ergosterol, cholesterol, and 7-, 20- and 25-hydroxy cholesterol. Moreira et al.(2001) refined the localization of the OSBP1 gene to chromosome 11q12.1 by radiation hybrid analysis. Wang et al.(2005) found that OSBP functions as a cholesterol-binding scaffolding protein coordinating the activity of 2 phosphatases to control the extracellular signal-regulated kinase (ERK) signaling pathway. Cytosolic OSBP formed an approximately 440-kD oligomer with a member of the PTPPBS family of tyrosine phosphatases, the serine/threonine phosphatase PP2A, and cholesterol.

UniProt: P22059