PARP1 antibody (AA 670-858)

Catalog No. ABIN3032162

Product Details anti-PARP1 Antibody

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Binding Specificity: AA 670-858

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purification: Antigen affinity

Immunogen: Human partial recombinant protein (AA 670-858) was used as the immunogen for this PARP antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the PARP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the PARP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP (PARP1 Products)

Synonyms: ADPRT antibody, ADPRT 1 antibody, ADPRT1 antibody, ARTD1 antibody, PARP antibody, PARP-1 antibody, PPOL antibody, pADPRT-1 antibody, Adprt antibody, Parp-1 antibody, 5830444G22Rik antibody, AI893648 antibody, Adprp antibody, Adprt1 antibody, C80510 antibody, parp-1 antibody, sPARP-1 antibody, BEST:LD21673 antibody, CG17685 antibody, CG17696 antibody, CG17718 antibody, CG40411 antibody, D.PARP antibody, Dm.pARTa antibody, Dmel\\CG40411 antibody, LD21673.3prime antibody, PARP1 antibody, dPARP antibody, parp antibody, adprt1 antibody, padprt-1 antibody, ppol antibody, si:dkey-206f10.3 antibody, wu:fc60f12 antibody, zgc:110092 antibody, poly(ADP-ribose) polymerase 1 antibody, poly (ADP-ribose) polymerase 1 antibody, poly (ADP-ribose) polymerase family, member 1 antibody, Poly-(ADP-ribose) polymerase antibody, poly(ADP-ribose) polymerase 1 L homeolog antibody, PARP1 antibody, Parp1 antibody, Parp antibody, parp1.L antibody, parp1 antibody

Background: Poly [ADP-ribose] polymerase 1 is composed of four domains: a DNA-binding domain, a caspase-cleaved domain, an auto-modification domain, and a catalytic domain. In vivo, PARP is cleaved by caspases -3 and -7 between aspartic acid 214 and glycine 215, resulting in a 24KD subunit (containing the DNA binding zinc finger motif) and a 89KD subunit (containing the catalytic and auto-modification domains).
PARP is an enzyme that modifies nuclear proteins. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage.

Gene ID: 142

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location