Peroxiredoxin 5 antibody (C-Term)

Catalog No. ABIN3032191

Product Details anti-Peroxiredoxin 5 Antibody

Target: Peroxiredoxin 5 (PRDX5)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Peroxiredoxin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human PRDX5 (DDSLVSIFGNRRLKRF) was used as the immunogen for this Peroxiredoxin 5 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Peroxiredoxin 5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Peroxiredoxin 5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Peroxiredoxin 5

Target: Peroxiredoxin 5 (PRDX5)

Abstract: PRDX5 Products

Synonyms: acr1 antibody, aoeb166 antibody, pmp20 antibody, prx-5 antibody, prx5 antibody, prxv antibody, sbbi10 antibody, GB16965 antibody, zgc:112318 antibody, PRDX5 antibody, CG 32920 antibody, CG 7217 antibody, CG32920 antibody, CG7217 antibody, Dmel\\CG7217 antibody, dPrx5 antibody, dprx5 antibody, ACR1 antibody, AOEB166 antibody, B166 antibody, PLP antibody, PMP20 antibody, PRDX6 antibody, PRXV antibody, prx-V antibody, AOPP antibody, Pmp20 antibody, Prdx6 antibody, PrxV antibody, Aoeb166 antibody, Prx V antibody, peroxiredoxin 5 L homeolog antibody, peroxiredoxin-5, mitochondrial antibody, peroxiredoxin 5 antibody, Peroxiredoxin 5 antibody, prdx5.L antibody, LOC552429 antibody, prdx5 antibody, PRDX5 antibody, Prx5 antibody, Prdx5 antibody

Background: Peroxiredoxin 5, also known as PRX5 and PRDX5, is a member of the peroxiredoxin family and may play an antioxidant protective role in various tissues under nonpathologic conditions and during inflammatory processes. PRDX5 displays mitochondrial presequence features and has 3 cysteines implicated in antioxidant activity and a C-terminal SQL peroxisomal targeting sequence. Northern blot analysis revealed ubiquitous expression of a 1.0-kb PRDX5 transcript in tissues and cell lines. Functional analysis showed that Peroxiredoxin 5 has antioxidant activity equivalent to that of CAT. While PRDX5 was localized to fibroblasts in normal tendon, it was localized to fibroblasts and endothelial cells in degenerative tendon. mRNA and protein levels increased at 12 hours, and the increase in PRDX5 expression correlated with reduced peroxide levels. Peroxiredoxin 5 plays a protective role against oxidative stress in human cartilage.

UniProt: P30044

Pathways: Cell RedoxHomeostasis