PRMT5 antibody (Protein Arginine Methyltransferase 5) (AA 76-104)

Details for Product anti-PRMT5 Antibody No. ABIN3032360
Antigen
Epitope
AA 76-104
19
15
11
10
7
6
5
4
3
2
1
1
1
1
1
Reactivity
Human
159
59
55
9
8
6
6
3
3
2
2
2
2
Host
Rabbit
144
10
8
Clonality
Polyclonal
Conjugate
This PRMT5 antibody is un-conjugated
10
7
7
5
5
5
4
4
4
4
4
4
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
120
77
55
22
13
13
11
4
4
3
3
3
2
1
Options
Immunogen A portion of amino acids 76-104 from the human protein was used as the immunogen for this PRMT5 antibody.
Isotype Ig Fraction
Cross-Reactivity (Details) Expected species reactivity: Bovine, Primate
Purification Antigen affinity purified
Antigen
Alternative Name PRMT5 (PRMT5 Antibody Abstract)
Background Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3), such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono-and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
UniProt O14744
Pathways Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development
Application Notes Titration of the PRMT5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
Restrictions For Research Use only
Format Liquid
Buffer In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Aliquot the PRMT5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Protein Arginine Methyltransferase 5 (PRMT5) (AA 76-104) antibody (ABIN3032360) PRMT5 antibody western blot analysis in HL-60 lysate.
Image no. 2 for anti-Protein Arginine Methyltransferase 5 (PRMT5) (AA 76-104) antibody (ABIN3032360) IHC testing of PRMT5 antibody and FFPE human testis tissue.
Image no. 3 for anti-Protein Arginine Methyltransferase 5 (PRMT5) (AA 76-104) antibody (ABIN3032360) PRMT5 antibody western blot analysis in HL-60 lysate.