RPS6KA5
Reactivity: Mouse, Rat
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
The stated application concentrations are suggested starting amounts. Titration of the MSK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
Restrictions
For Research Use only
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage
-20 °C
Storage Comment
After reconstitution, the MSK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Target
MSK1 (RPS6KA5)
(Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))
Ribosomal protein S6 kinase alpha-5, also known as MSK1, is an enzyme that in humans is encoded by the RPS6KA5 gene. It belongs to the AGC family of kinases. MSK1 gene is mapped to chromosome 14q31-q32. Northern blot analysis indicated that MSK1 was expressed as a 4-kb mRNA in all tissues tested, with the highest levels of expression in brain, muscle, and placenta. MSK1 was activated in vitro and in vivo by either ERK or SAPK2 proteins. It has been found that MSK1, rather than MAPKAP-K1 or MAPKAP-K2/K3, mediates activation of the cAMP response element-binding protein and activating transcription factor-1 by either growth factors or stress signals.