GLUT4 antibody (AA 491-509)

Catalog No. ABIN3032677

This Rabbit Polyclonal antibody specifically detects GLUT4 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GLUT4 antibody (AA 491-509) (ABIN3032677)

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GLUT4 Antibody

Binding Specificity: AA 491-509

Purification: Antigen affinity

Immunogen: Amino acids 491-509 (EQEVKPSTELEYLGPDEND) were used as the immunogen for this GLUT4 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the GLUT4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the GLUT4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for GLUT4

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Alternative Name: GLUT4 (Glucose Transporter 4)

Background: Facilitated glucose transport by mammalian cells is not a property of a single protein but an activity associated with a family of structurally related proteins. Glucose transporter 4 is a insulin-responsive glucose transporter. It belongs to solute carrier family 2, member 1. Insulin alters the subcellular localization of GLUT4 vesicles in human muscle, and that this effect is impaired equally in insulin-resistant subjects with and without diabetes. A similar pattern of defects cause insulin resistance in human adipocytes. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

UniProt: P14672

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Proton Transport, Brown Fat Cell Differentiation, Warburg Effect