COL4a2 antibody (C-Term)

Catalog No. ABIN3042317

Product Details anti-COL4a2 Antibody

Target: COL4a2 (COL4A2) (Collagen, Type IV, alpha 2 (COL4A2))

Binding Specificity: AA 1697-1712, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL4a2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Collagen alpha-2(IV) chain(COL4A2) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Sequence: LIRTHISRCQ VCMKNL

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Collagen alpha-2(IV) chain(COL4A2) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: collagen, type IV, alpha 2
Protein Name: Collagen alpha-2(IV) chain

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen IV(1697-1712aa LIRTHISRCQVCMKNL), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-COL4a2 antibody (ABIN3042317)

Zhu, Chen, Chen, Yang, Xie: "Astaxanthin Promotes Nrf2/ARE Signaling to Alleviate Renal Fibronectin and Collagen IV Accumulation in Diabetic Rats." in: Journal of diabetes research, Vol. 2018, pp. 6730315, (2018) (PubMed).

Luo, Deng, Lamsal, Xu, Xiang, Cheng: "AMP-activated protein kinase alleviates extracellular matrix accumulation in high glucose-induced renal fibroblasts through mTOR signaling pathway." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 35, Issue 1, pp. 191-200, (2015) (PubMed).

Qiu, Zhang, Zhao, Zhang, Wu, Guo, Hu: "Enhancement of endothelial differentiation of adipose derived mesenchymal stem cells by a three-dimensional culture system of microwell." in: Biomaterials, Vol. 53, pp. 600-8, (2015) (PubMed).

Liu, Lu, Li, Wei, Li: "Engineering blood vessels through micropatterned co-culture of vascular endothelial and smooth muscle cells on bilayered electrospun fibrous mats with pDNA inoculation." in: Acta biomaterialia, Vol. 11, pp. 114-25, (2014) (PubMed).

Ji, Wu, Ma, Ma, Qin: "The effect of resveratrol on the expression of AdipoR1 in kidneys of diabetic nephropathy." in: Molecular biology reports, Vol. 41, Issue 4, pp. 2151-9, (2014) (PubMed).

Wenbin, Guojun: "Resveratrol Ameliorates Diabetes-induced Renal Damage through Regulating the Expression of TGF-?1, Collagen IV and Th17/Treg-related Cytokines in Rats." in: The West Indian medical journal, Vol. 63, Issue 1, pp. 20-5, (2014) (PubMed).

Chen, Wan, Xia, Guo, Wang, Liu, Li: "Promoted regeneration of mature blood vessels by electrospun fibers with loaded multiple pDNA-calcium phosphate nanoparticles." in: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fu?r Pharmazeutische Verfahrenstechnik e.V, Vol. 85, Issue 3 Pt A, pp. 699-710, (2013) (PubMed).

Xu, Zheng, He, Li, Zhang, Liang: "Puerarin, isolated from Pueraria lobata (Willd.), protects against hepatotoxicity via specific inhibition of the TGF-?1/Smad signaling pathway, thereby leading to anti-fibrotic effect." in: Phytomedicine : international journal of phytotherapy and phytopharmacology, Vol. 20, Issue 13, pp. 1172-9, (2013) (PubMed).

Wen, Huang, Zhang, Zhang, Chen, Gu, Hao: "Resveratrol attenuates diabetic nephropathy via modulating angiogenesis." in: PLoS ONE, Vol. 8, Issue 12, pp. e82336, (2013) (PubMed).

He, Xia, Wang, Wei, Luo, Li: "Multiple release of polyplexes of plasmids VEGF and bFGF from electrospun fibrous scaffolds towards regeneration of mature blood vessels." in: Acta biomaterialia, Vol. 8, Issue 7, pp. 2659-69, (2012) (PubMed).

Qi, Kai-Chen, Liu, Su, Yu: "Protective effect of Icariin on the early stage of experimental diabetic nephropathy induced by streptozotocin via modulating transforming growth factor ?1 and type IV collagen expression in rats." in: Journal of ethnopharmacology, Vol. 138, Issue 3, pp. 731-6, (2011) (PubMed).

Zhang, Wu, Zheng, Li: "Adenovirus-mediated decorin gene transfection has therapeutic effects in a streptozocin-induced diabetic rat model." in: Nephron. Experimental nephrology, Vol. 116, Issue 1, pp. e11-21, (2010) (PubMed).

Xue, Zhang, Liu, Wang: "A new ATP-sensitive potassium channel opener protects the kidney from hypertensive damage in spontaneously hypertensive rats." in: The Journal of pharmacology and experimental therapeutics, Vol. 315, Issue 2, pp. 501-9, (2005) (PubMed).

Target Details for COL4a2

Target: COL4a2 (COL4A2) (Collagen, Type IV, alpha 2 (COL4A2))

Alternative Name: COL4A2 (COL4A2 Products)

Background: Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.

Synonyms: Arresten antibody|Canstatin antibody|CO4A1_HUMAN antibody|COL4A1 antibody|COL4A1 NC1 domain antibody|COL4A2 antibody|COL4A3 antibody|COL4A4 antibody|COL4A5 antibody|Collagen Alpha 1(IV) Chain antibody|Collagen Alpha 2(IV) Chain antibody|collagen alpha-1(IV) chain antibody|Collagen IV Alpha 1 Polypeptide antibody|Collagen IV Alpha 2 Polypeptide antibody|Collagen Of Basement Membrane Alpha 1 Chain antibody|Collagen Of Basement Membrane Alpha 2 Chain antibody|Collagen Type IV Alpha 1 antibody|Collagen Type IV Alpha 2 antibody|Collagen Type IV Alpha 3 antibody|Collagen Type IV Alpha 4 antibody|Collagen Type IV Alpha 5 antibody|DKFZp686I14213 antibody|FLJ22259 antibody

UniProt: P08572