GAD antibody (C-Term)

Catalog No. ABIN3042597

Product Details anti-GAD Antibody

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Binding Specificity: AA 529-543, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAD antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Glutamate decarboxylase 1(GAD1) detection. Tested with WB in Human,Mouse,Rat.

Sequence: PQRREKLHKV APKIK

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Glutamate decarboxylase 1(GAD1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: glutamate decarboxylase 1(brain, 67 kDa)
Protein Name: Glutamate decarboxylase 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GAD67(529-543aa PQRREKLHKVAPKIK), different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-GAD antibody (ABIN3042597)

Yang, Wang, He, Ding, Sun, Hua, Xi: "Dietary Restriction Affects Neuronal Response Property and GABA Synthesis in the Primary Visual Cortex." in: PLoS ONE, Vol. 11, Issue 2, pp. e0149004, (2016) (PubMed).

Target Details for GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Alternative Name: GAD1 (GAD1 Products)

Synonyms: CPSQ1 antibody, GAD antibody, SCP antibody, GAD-67 antibody, cpsq1 antibody, gad1 antibody, gad1-A antibody, gad67 antibody, GAD67 antibody, GAD1 antibody, GLUTAMATE DECARBOXYLASE antibody, GLUTAMATE DECARBOXYLASE 1 antibody, MKP11.30 antibody, MKP11_30 antibody, glutamate decarboxylase antibody, EP10 antibody, GAD25 antibody, GAD44 antibody, Gad-1 antibody, Gad67 antibody, glutamate decarboxylase 1 antibody, glutamate decarboxylase 1 L homeolog antibody, glutamate decarboxylase antibody, glutamate decarboxylase 1b antibody, GAD1 antibody, gad1.1.L antibody, GAD antibody, Gad1 antibody, gad1b antibody

Background: Glutamic acid decarboxylase(GAD) catalyses the conversion of L-glutamic acid to the inhibitory neurotransmitter gamma-aminobutyric acid(GABA). Two forms of human GAD, GAD65 and GAD67, are encoded by two separate genes. Human GAD65 cDNA encodes a Mr 65,000 polypeptide, with 585 amino acid residues, whereas human GAD67 encodes a Mr 67,000 polypeptide, with 594 amino acid residues. GAD67 gene consists of 16 exons, spread over more than 45 kb of genomic DNA. The GAD67 gene contains an additional exon(exon 0) that together with part of exon 1, specifies the 5' untranslated region of GAD67 mRNA. Human GAD67 shows 65 % identity to GAD65 and is located in 2q31. GAD67 may play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures.

Synonyms: 67 kDa glutamic acid decarboxylase antibody|DCE1 antibody|DCE1_HUMAN antibody|EC 4.1.1.15 antibody|FLJ45882 antibody|GAD 1 antibody|GAD 67 antibody|GAD antibody|GAD-67 antibody|GAD1 antibody|GADA antibody|Glutamate decarboxylase 1(brain 67kD) antibody|Glutamate decarboxylase 1 antibody|Glutamate decarboxylase 1 brain 67kD antibody|Glutamate decarboxylase 1 brain 67 kDa antibody|Glutamate decarboxylase 67 kDa isoform antibody|OTTHUMP00000041055 antibody, SCP antibody

UniProt: Q99259