PTTG1 antibody (C-Term)
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- Target See all PTTG1 Antibodies
- PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))
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Binding Specificity
- AA 146-161, C-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PTTG1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Purpose
- Rabbit IgG polyclonal antibody for Securin(PTTG1) detection. Tested with WB in Human.
- Sequence
- LMILDEEREL EKLFQL
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Securin(PTTG1) detection. Tested with WB in Human.
Gene Name: pituitary tumor-transforming 1
Protein Name: Securin - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-terminus of human Securin(146-161aa LMILDEERELEKLFQL), different from the related rat sequence by four amino acids, and from the related mouse sequence by five amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product PTTG1 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Thimerosal (Merthiolate), Sodium azide
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Expiry Date
- 12 months
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Pituitary tumor-transforming gene 1 (PTTG1) is overexpressed in oral squamous cell carcinoma (OSCC) and promotes migration, invasion and epithelial-mesenchymal transition (EMT) in SCC15 cells." in: Tumour biology, Vol. 35, Issue 9, pp. 8801-11, (2014) (PubMed).
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Pituitary tumor-transforming gene 1 (PTTG1) is overexpressed in oral squamous cell carcinoma (OSCC) and promotes migration, invasion and epithelial-mesenchymal transition (EMT) in SCC15 cells." in: Tumour biology, Vol. 35, Issue 9, pp. 8801-11, (2014) (PubMed).
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- Target
- PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))
- Alternative Name
- PTTG1 (PTTG1 Products)
- Synonyms
- EAP1 antibody, HPTTG antibody, PTTG antibody, TUTR1 antibody, PTTG1 antibody, securin antibody, AW555095 antibody, C87862 antibody, Pttg antibody, Pttg3 antibody, Securin antibody, Protein Cut2 antibody, pituitary tumor-transforming 1 antibody, pituitary tumor-transforming gene 1 antibody, securin, sister chromatid separation inhibitor antibody, PTTG1 antibody, Pttg1 antibody, cut2 antibody
- Background
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Securin, also known as pituitary tumor-transforming 1 or EAP1 is a protein that in humans is encoded by the PTTG1 gene. PTTG1 gene is mapped to 5q33.3. The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex(APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box(D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.
Synonyms: EAP1 antibody|PTTG antibody|TUTR1 antibody|Hpttg antibody|TUMOR-TRANSFORMING 1 antibody|Esp1-associated protein antibody|ESP1-ASSOCIATED PROTEIN 1 antibody|Tumor-transforming protein 1 antibody|Pituitary tumor-transforming gene 1 protein antibody - UniProt
- O95997
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