PIK3R1 antibody (N-Term)

Catalog No. ABIN3042707

Product Details anti-PIK3R1 Antibody

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Binding Specificity: AA 65-80, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Phosphatidylinositol 3-kinase regulatory subunit alpha(PIK3R1) detection. Tested with WB in Human,Mouse,Rat.

Sequence: ERGDFPGTYV EYIGRK

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Phosphatidylinositol 3-kinase regulatory subunit alpha(PIK3R1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: phosphoinositide-3-kinase, regulatory subunit 1(alpha)
Protein Name: Phosphatidylinositol 3-kinase regulatory subunit alpha(PI3-kinase regulatory subunit alpha/PI3K regulatory subunit alpha/PtdIns-3-kinase regulatory subunit alpha)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PI 3 Kinase p85 alpha(65-80aa ERGDFPGTYVEYIGRK), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-PIK3R1 antibody (ABIN3042707)

Yin, Chang, Xu: "Expressions Profiles of the Proteins Associated with Carbohydrate Metabolism in Rat Liver Regeneration." in: BioMed research international, Vol. 2017, pp. 8428926, (2018) (PubMed).

Target Details for PIK3R1

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Alternative Name: PIK3R1 (PI3K p85a Products)

Synonyms: PI3CG antibody, PI3K antibody, PI3Kgamma antibody, PIK3 antibody, AGM7 antibody, GRB1 antibody, p85 antibody, p85-ALPHA antibody, PI3KA antibody, AA414921 antibody, C530050K14 antibody, p50alpha antibody, p55alpha antibody, p85alpha antibody, wu:fb65b05 antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma antibody, phosphoinositide-3-kinase regulatory subunit 1 antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta antibody, phosphoinositide-3-kinase, regulatory subunit 1 (alpha) antibody, PIK3CG antibody, PIK3R1 antibody, Pik3r1 antibody, Pik3cb antibody, pik3r1 antibody

Background: Phosphatidylinositol 3-kinase regulatory subunit alpha is an enzyme that in humans is encoded by the PIK3R1 gene. Its gene is mapped to 5q13. the bovine PI3K p85 subunit consists of 2 closely related proteins, p85-alpha and p85-beta. They cloned cDNAs encoding both p85 subunits, each of which is a 724-amino acid polypeptide. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.

Synonyms: GRB 1 antibody|GRB1 antibody|p85 alpha antibody|p85 antibody|p85a antibody|P85A_HUMAN antibody|Phosphatidylinositol 3 kinase associated p 85 alpha antibody|Phosphatidylinositol 3 kinase associated p85 alpha antibody|Phosphatidylinositol 3 kinase regulatory 1 antibody|Phosphatidylinositol 3 kinase regulatory alpha subunit antibody|Phosphatidylinositol 3 kinase regulatory subunit alpha antibody|Phosphatidylinositol 3 kinase regulatory subunit polypeptide 1 antibody|Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1(p85 alpha) antibody|Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody|Phosphatidylinositol 3-kinase regulatory subunit alpha antibody|Phosphoinositide 3 kinase regulatory subunit 1 antibody|Phosphoinositide 3 kinase regulatory subunit polypeptide 1(p85 alpha) antibody|Phosphoinositide 3 kinase regulatory subunit polypeptide 1 antibody|Phosphoinositide 3 kinase, regulatory subunit 1(alpha) antibody|PI3 kinase p85 alpha subunit antibody|PI3 kinase p85 subunit alpha antibody|PI3-kinase regulatory subunit alpha antibody|PI3-kinase subunit p85-alpha antibody|PI3K antibody|PI3K regulatory subunit alpha antibody|PIK3R 1 antibody|PIK3R1 antibody|PtdIns 3 kinase p85 alpha antibody|PtdIns-3-kinase regulatory subunit alpha antibody|PtdIns-3-kinase regulatory subunit p85-alpha antibody

UniProt: P27986

Pathways: TCR Signaling, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, Warburg Effect