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CD47 antibody (CD47) (AA 308-323) Primary Antibody

CD47 Reactivity: Human, Rat IHC (p), WB Host: Rabbit Polyclonal
Catalog No. ABIN3042825
$280.00
Plus shipping costs $45.00
100 μg
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  • Target
    Binding Specificity
    AA 308-323, C-Term
    Reactivity
    Human, Rat
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    This CD47 antibody is un-conjugated
    Application
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Purpose
    Rabbit IgG polyclonal antibody for Leukocyte surface antigen CD47(CD47) detection. Tested with WB, IHC-P in Human,Rat.
    Sequence
    EPLNAFKESK GMMNDE
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Leukocyte surface antigen CD47(CD47) detection. Tested with WB, IHC-P in Human,Rat.
    Gene Name: CD47 Molecule
    Protein Name: Leukocyte surface antigen CD47
    Purification
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the C-terminus of human CD47(308-323aa EPLNAFKESKGMMNDE), identical to the related rat sequence.
    Isotype
    IgG
  • Target
    Alternative Name
    CD47 (CD47 Antibody Abstract)
    Background
    CD47, also known as IAP or MER6, is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 gene belongs to the immunoglobulin superfamily. This gene is mapped to 3q13.12. CD47 gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes.

    Synonyms: Antigenic surface determinant protein OA3 antibody|CD 47 antibody|CD47 antibody|Cd47 antibody|CD47 antigen(Rh-related antigen, integrin-associated signal transducer) antibody|CD47 antigen antibody|CD47 glycoprotein antibody|CD47 Molecule antibody|CD47_HUMAN antibody|IAP antibody|Integrin Associated Protein antibody|Integrin associated signal transducer antibody|Integrin-associated protein antibody|Leukocyte surface antigen CD47 antibody|MER 6 antibody|MER6 antibody|OA 3 antibody|OA3 antibody|OTTHUMP00000041152 antibody|OTTHUMP00000041153 antibody|Protein MER6 antibody|Rh related antigen antibody
    UniProt
    Q08722
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Preservative
    Thimerosal (Merthiolate), Sodium azide
    Precaution of Use
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    Expiry Date
    12 months
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