PAD4 antibody (Middle Region)

Catalog No. ABIN3042934

Product Details anti-PAD4 Antibody

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Binding Specificity: AA 492-512, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAD4 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Protein-arginine deiminase type-4(PADI4) detection. Tested with WB in Human.

Sequence: ASPRSCYKLF QEQQNEGHGE A

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Protein-arginine deiminase type-4(PADI4) detection. Tested with WB in Human.
Gene Name: peptidyl arginine deiminase, type IV
Protein Name: Protein-arginine deiminase type-4

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PADI4(492-512aa ASPRSCYKLFQEQQNEGHGEA).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for PADI4 is approximately 0.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PAD4

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Alternative Name: PADI4 (PADI4 Products)

Synonyms: PAD antibody, PAD4 antibody, PADI5 antibody, PDI4 antibody, PDI5 antibody, Pad4 antibody, Pdi4 antibody, peptidyl arginine deiminase 4 antibody, peptidyl arginine deiminase, type IV antibody, PADI4 antibody, Padi4 antibody

Background: PADI4(Peptidylarginine Deiminase, Type IV), also known as PAD or PAD4, is a human protein which in humans is encoded by the PADI4 gene. Peptidylarginine deiminases, such as PADI4, make up a family of posttranslational modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ion(Nakashima et al., 1999). Suzuki et al.(2003) found from sequence data that the PADI4 gene maps to 1p36. Cuthbert et al.(2004) described a process, deimination, that converts histone arginine to citrulline and antagonizes arginine methylation. They showed that PADI4 specifically deiminated arg2, arg8, arg17, and arg26 in the H3 tail. Deimination by PADI4 prevented arginine methylation by CARM1. Dimethylation of arginines prevented deimination by PADI4, although monomethylation still allowed deimination to take place. In vivo targeting experiments on an endogenous promoter demonstrated that PADI4 could repress hormone receptor-mediated gene induction. PADI4 was recruited to the pS2 promoter following hormone induction when the gene was transcriptionally downregulated, consistent with a repressive role for PADI4.

Synonyms: EC 3.5.3.15 antibody|HL 60 PAD antibody|HL-60 PAD antibody|PAD 4 antibody|PAD antibody|PADI 4 antibody|PADI 5 antibody|PADI H protein antibody|Padi4 antibody|PADI4_HUMAN antibody|PADI5 antibody|PDI 4 antibody|PDI 5 antibody|PDI4 antibody|PDI5 antibody|Peptidyl arginine deiminase type IV antibody|Peptidyl arginine deiminase type V antibody|Peptidylarginine deiminase IV antibody|Protein arginine deiminase antibody|Protein arginine deiminase type 4 antibody|Protein arginine deiminase type IV antibody|Protein-arginine deiminase type IV antibody|Protein-arginine deiminase type-4 antibody