TNFRSF10A antibody (N-Term)

Catalog No. ABIN3042972

Product Details anti-TNFRSF10A Antibody

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Binding Specificity: AA 110-125, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFRSF10A antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 10A(TNFRSF10A) detection. Tested with WB in Human.

Sequence: TIKLHDQSIG TQQWEH

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 10A(TNFRSF10A) detection. Tested with WB in Human.
Gene Name: tumor necrosis factor receptor superfamily, member 10a
Protein Name: Tumor necrosis factor receptor superfamily member 10A

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human DR4(110-125aa TIKLHDQSIGTQQWEH).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for TNFRSF10A is approximately 1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-TNFRSF10A antibody (ABIN3042972)

Liu, Zuo, Wang, Chen, Yang, Wang, Zhu: "miR-942 decreases TRAIL-induced apoptosis through ISG12a downregulation and is regulated by AKT." in: Oncotarget, Vol. 5, Issue 13, pp. 4959-71, (2014) (PubMed).

Fu, Ren, Wang, Fei, Wang, Wang: "DJ-1 inhibits TRAIL-induced apoptosis by blocking pro-caspase-8 recruitment to FADD." in: Oncogene, Vol. 31, Issue 10, pp. 1311-22, (2012) (PubMed).

Zhang, Niu, Yang, Lu, Chen: "The occurrence and regional distribution of DR4 on herniated disc cells: a potential apoptosis pathway in lumbar intervertebral disc." in: Spine, Vol. 33, Issue 4, pp. 422-7, (2008) (PubMed).

Target Details for TNFRSF10A

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Alternative Name: TNFRSF10A (TNFRSF10A Products)

Synonyms: APO2 antibody, CD261 antibody, DR4 antibody, TRAILR-1 antibody, TRAILR1 antibody, TNFRSF10A antibody, TNF receptor superfamily member 10a antibody, tumor necrosis factor receptor superfamily member 26 antibody, tumor necrosis factor receptor superfamily, member 10a antibody, TNFRSF10A antibody, LOC510798 antibody

Background: TNFRSF10A(Tumor Necrosis Factor Receptor Subfamily Member 10A), also known as APO2, DR4 or TRAILR1, is a protein that in humans is encoded by the TNFRSF10A gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. By analysis of radiation hybrids, Marsters et al.(1997) mapped the DR4 gene to 8p21. The TRAIL receptor DR5, and 2 decoy receptors for TRAIL, DCR1, and DCR2, are located in the same region, suggesting that these receptors arose from recent gene duplication events. Pan et al.(1997) found that, as with FAS, TNFR1, and DR3, overexpression of DR4 induced apoptosis. However, unlike the other 3 death receptors, DR4 did not use FADD to transmit the death signal, suggesting the use of distinct proximal signaling machinery.

Synonyms: Apo 2 antibody|Apo2 antibody|CD 261 antibody|CD261 antibody|CD261 antigen antibody|Cytotoxic ligand TRAIL receptor antibody|Cytotoxic TRAIL receptor antibody|Death receptor 4 antibody|DR 4 antibody|DR4 antibody|MGC9365 antibody|NF related apoptosis-inducing ligand receptor 1 antibody|T10A antibody|TNF related apoptosis inducing ligand receptor 1 antibody|TNF-related apoptosis-inducing ligand receptor 1 antibody|TNFRSF10A antibody|TR10A_HUMAN antibody|TRAIL R1 antibody|TRAIL receptor 1 antibody|TRAIL-R1 antibody|TRAILR 1 antibody|TRAILR1 antibody|Tumor necrosis factor receptor superfamily member 10a antibody|Tumor necrosis factor receptor superfamily member 10A precursor antibody

UniProt: O00220

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity