STAT5A antibody (C-Term)

Catalog No. ABIN3043020

Product Details anti-STAT5A Antibody

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Binding Specificity: AA 735-751, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT5A antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 5A(STAT5A) detection. Tested with WB in Human.

Sequence: YNMYPQNPDH VLDQDGE

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 5A(STAT5A) detection. Tested with WB in Human.
Gene Name: signal transducer and activator of transcription 5A
Protein Name: Signal transducer and activator of transcription 5A

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human STAT5a(735-751aa YNMYPQNPDHVLDQDGE).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-STAT5A antibody (ABIN3043020)

Liu, Li, Liang, Li, Jiang, Chu, Yang: "Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway." in: International journal of molecular medicine, Vol. 41, Issue 4, pp. 1867-1876, (2018) (PubMed).

Hu, Zhang, Tang, Su, Li, Chen, Zhang, Cai, Zhu: "Variant G6PD levels promote tumor cell proliferation or apoptosis via the STAT3/5 pathway in the human melanoma xenograft mouse model." in: BMC cancer, Vol. 13, Issue 1, pp. 251, (2014) (PubMed).

Target Details for STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Alternative Name: STAT5A (STAT5A Products)

Synonyms: MGF antibody, STAT5 antibody, AA959963 antibody, Stat5 antibody, STAT5A/MGF antibody, STATA5 antibody, STAT5A antibody, stat5 antibody, stat5b antibody, signal transducer and activator of transcription 5A antibody, signal transducer and activator of transcription 5a antibody, STAT5A antibody, Stat5a antibody, stat5a antibody, LOC100348518 antibody, LOC100716811 antibody

Background: STAT5(Signal transducer and activator of transcription 5) also known as STAT5A, MGF, is a protein that serve the dual function of signal transducers and activators of transcription in cells exposed to signaling polypeptides. Hou et al.(1995) cloned the human STAT5 cDNA from an umbilical vein endothelial cell library and found that it encodes a 794-amino acid polypeptide with a predicted mass of approximately 90.5 kD. To analyze the possible role of STAT5 in human GH-induced proliferation, Friedrichsen et al.(2001) expressed a dominant-negative STAT5 mutant, STAT5A-delta-749, in INS-1 cells under the control of a doxycycline-inducible promoter by stable transfection. Chromatin immunoprecipitation analysis confirmed direct binding of STAT5 to the HIF2A promoter 344 bp upstream of the HIF2A start site. STAT5 overexpression independently resulted in erythroid commitment in megakaryocytic-erythroid progenitors, which was abrogated by knockdown of GATA1. STAT5 is responsible for the immediate-early induction of the long isoform of the BCL2-related protein in erythroid cells through direct binding to the promoter of the BCLX gene.

Synonyms: Mammary gland factor antibody|MGF antibody|Signal transducer and activator of transcription 5A antibody|STA5A_HUMAN antibody|STAT 5 antibody|STAT 5A antibody|STAT5 antibody|STAT5A antibody

UniProt: P42229

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation