AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 461-477)

Details for Product anti-AKT1 Antibody No. ABIN3043108
Antigen
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Alternatives
anti-Rat (Rattus) AKT antibody for Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)
Epitope
AA 461-477
181
92
85
72
50
50
44
30
29
25
24
24
19
18
17
17
15
14
13
13
13
11
11
10
10
10
10
9
9
8
7
7
7
6
6
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
1320
741
618
55
53
53
34
33
30
28
6
6
5
4
4
3
3
2
1
1
1
1
Host
Mouse
1096
256
11
4
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This AKT antibody is un-conjugated
63
37
33
32
29
26
19
19
19
19
18
18
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Western Blotting (WB)
1100
612
596
225
162
135
135
59
54
34
32
18
13
11
8
8
5
3
3
2
1
1
1
1
1
Options
Purpose Mouse IgG monoclonal antibody for PKB alpha, v-akt murine thymoma viral oncogene homolog 1 (AKT1) detection. Tested with WB in Human,mouse,rat,chicken. No cross reactivity with other proteins.
Immunogen Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH.
Clone PKB-175
Isotype IgG1
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Mouse IgG monoclonal antibody for PKB alpha, v-akt murine thymoma viral oncogene homolog 1 (AKT1) detection. Tested with WB in Human,mouse,rat,chicken.
Gene Name: v-akt murine thymoma viral oncogene homolog 1
Protein Name: RAC-alpha serine/threonine-protein kinase
Purification Ascites
Plasmids, Primers & others Plasmids, Primers & others AKT products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background PKB alpha also knows as V-AKT murine thymoma vial oncogene homolog 1(ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. AKT1 gene is mapped to chromosome 14q32.3. Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo.

Synonyms: AKT|PKB|RAC|CWS6|PRKBA|PKB-ALPHA|RAC-ALPHA|ONCOGENE AKT1|PROTEIN KINASE B-ALPHA|RAC SERINE/THREONINE PROTEIN KINASE|Protein kinase B|Proto-oncogene c-Akt|RAC-PK-alpha
Gene ID 207, 11651, 24185
UniProt P31749, P31750, P47196
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling
Application Notes WB: Concentration: 0.25-0.5 μg/mL, Species: Human, mouse, rat, chicken
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921123 in WB.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 1 mL of PBS buffer will yield a concentration of 100 μg/mL.
Concentration 100 μg/mL
Buffer Mouse ascites fluid, 1.2 % sodium acetate, 2 mg BSA, with 0.01 mg Sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Supplier Images
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 461-477) antibody (ABIN3043108) Anti- PKB alpha antibody, Western blotting All lanes: Anti PKB alpha at 0.5ug/ml Lan...
Product cited in: Wan, Wu: "Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways." in: Journal of experimental & clinical cancer research : CR, Vol. 35, Issue 1, pp. 119, 2017 (PubMed).

Liu, Gao, Wang, Zhang, Xu, Lin, Wang, Wu, Guo: "Catalpol promotes cellular apoptosis in human HCT116 colorectal cancer cells via microRNA-200 and the downregulation of PI3K-Akt signaling pathway." in: Oncology letters, Vol. 14, Issue 3, pp. 3741-3747, 2017 (PubMed).

Zhang, Wu, Huang: "Epicatechin Plus Treadmill Exercise are Neuroprotective Against Moderate-stage Amyloid Precursor Protein/Presenilin 1 Mice." in: Pharmacognosy magazine, Vol. 12, Issue Suppl 2, pp. S139-46, 2016 (PubMed).

Ma, Zhou, Li, Zheng, Wu, Niu, Tang, He, Li, Hua: "Lin28a promotes self-renewal and proliferation of dairy goat spermatogonial stem cells (SSCs) through regulation of mTOR and PI3K/AKT." in: Scientific reports, Vol. 6, pp. 38805, 2016 (PubMed).

Wan, Che, Kang, Wu: "SOCS3 blocks HIF-1? expression to inhibit proliferation and angiogenesis of human small cell lung cancer by downregulating activation of Akt, but not STAT3." in: Molecular medicine reports, Vol. 12, Issue 1, pp. 83-92, 2015 (PubMed).

Tang, Zhang, Qi, Chen, Li, Liu, He, Li, Zu: "Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line." in: Experimental and therapeutic medicine, Vol. 9, Issue 5, pp. 1851-1856, 2015 (PubMed).

Li, Zhang, Chai, Wang: "Progesterone alleviates hypoxic-ischemic brain injury via the Akt/GSK-3β signaling pathway." in: Experimental and therapeutic medicine, Vol. 8, Issue 4, pp. 1241-1246, 2014 (PubMed).

Wang, Sun, Li, Dong, Li, Zhao: "Resveratrol attenuates intermittent hypoxia-induced insulin resistance in rats: involvement of Sirtuin 1 and the phosphatidylinositol-4,5-bisphosphate 3-kinase/AKT pathway." in: Molecular medicine reports, Vol. 11, Issue 1, pp. 151-8, 2014 (PubMed).

Wang, Li, Lu, Zhao, Xu: "Taurine attenuates oxidative stress and alleviates cardiac failure in type I diabetic rats." in: Croatian medical journal, Vol. 54, Issue 2, pp. 171-9, 2013 (PubMed).

Qin, Niu, Wang, Xu, Qiao, Gu: "Heparanase induced by advanced glycation end products (AGEs) promotes macrophage migration involving RAGE and PI3K/AKT pathway." in: Cardiovascular diabetology, Vol. 12, pp. 37, 2013 (PubMed).

Wang, Li, Lu, Bao, Zhao: "Luteolin ameliorates cardiac failure in type I diabetic cardiomyopathy." in: Journal of diabetes and its complications, Vol. 26, Issue 4, pp. 259-65, 2012 (PubMed).

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