IL-18 antibody (AA 36-192)

Catalog No. ABIN3043244

Product Details anti-IL-18 Antibody

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Binding Specificity: AA 36-192

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-18 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Interleukin-18(IL18) detection. Tested with WB in Mouse.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Interleukin-18(IL18) detection. Tested with WB in Mouse.
Gene Name: interleukin 18
Protein Name: Interleukin-18

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse IL-18 recombinant protein (Position: N36-S192). Mouse IL-18 shares 66% and 91% amino acid (aa) sequence identity with human and rat IL-18, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, The detection limit for IL-18 is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-IL-18 antibody (ABIN3043244)

Si, Zhang, Gao, Chen, Zhao, Cheng: "Effects of IFN-? on IL-18 Expression in Pregnant Rats and Pregnancy Outcomes." in: Asian-Australasian journal of animal sciences, Vol. 26, Issue 10, pp. 1399-405, (2014) (PubMed).

Wang, Zhang, Zhang, Xu, Fang: "Expression and localization of IL-18 in the hypothalamic-pituitary-ovarian axis of non-pregnant, pregnant, and abortive rats." in: Journal of reproductive immunology, Vol. 92, Issue 1-2, pp. 45-53, (2011) (PubMed).

Yang, Shen, Chen, Ge: "Role of interleukin 18 in acute lung inflammation induced by gut ischemia reperfusion." in: World journal of gastroenterology, Vol. 11, Issue 29, pp. 4524-9, (2005) (PubMed).

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: IL18 (IL18 Products)

Synonyms: Igif antibody, Il-18 antibody, IL-18 antibody, IGIF antibody, ChIL-18 antibody, IL-1g antibody, IL1F4 antibody, IL18 antibody, interleukin 18 antibody, Il18 antibody, IL18 antibody

Background: Interleukin-18, also known as IL18, is a protein which in humans is encoded by the IL18 gene. IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. It is mapped to 11q22.2-q22.3. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12, it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells.

Synonyms: Iboctadekin antibody|IFN gamma inducing factor antibody|IFN-gamma-inducing factor antibody|IGIF antibody|IL 1 gamma antibody|IL 18 antibody|IL 1g antibody|IL-1 gamma antibody|IL-18 antibody|IL1 gamma antibody|IL18 antibody|IL18 protein antibody|IL18_HUMAN antibody|IL1F4 antibody|IL1g antibody|IL1gamma antibody|ILIF4 antibody|Interferon gamma inducing factor antibody|Interferon gamma-inducing factor antibody|Interleukin 1 gamma antibody|Interleukin 18 antibody|Interleukin-1 gamma antibody|Interleukin-18 antibody|Interleukin18 antibody|MGC12320 antibody

Gene ID: 16173

UniProt: P70380

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome