SEMA3A antibody (AA 550-771)

Catalog No. ABIN3043328

Product Details anti-SEMA3A Antibody

Target: SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))

Binding Specificity: AA 550-771

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEMA3A antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Semaphorin-3A(SEMA3A) detection. Tested with WB in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Semaphorin-3A(SEMA3A) detection. Tested with WB in Human.
Gene Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Protein Name: Semaphorin-3A

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Semaphorin 3A recombinant protein (Position: K550-V771). Human Semaphorin 3A shares 93% and 94% amino acid (aa) sequence identity with mouse and rat Semaphorin 3A, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Semaphorin 3A is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-SEMA3A antibody (ABIN3043328)

Zhou, Wu, Fu, Lv, Zhang: "Significance of semaphorin-3A and MMP-14 protein expression in non-small cell lung cancer." in: Oncology letters, Vol. 7, Issue 5, pp. 1395-1400, (2014) (PubMed).

Target Details for SEMA3A

Target: SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))

Alternative Name: SEMA3A (SEMA3A Products)

Synonyms: COLL1 antibody, HH16 antibody, Hsema-I antibody, Hsema-III antibody, SEMA1 antibody, SEMAD antibody, SEMAIII antibody, SEMAL antibody, SemD antibody, coll-1 antibody, Semad antibody, COLL-1 antibody, SEMA3A antibody, Dsema3a antibody, Sema3A antibody, sema1a antibody, sema3a1 antibody, semaZ1a antibody, sema1b antibody, sema3a2 antibody, semaZ1b antibody, semaphorin 3A antibody, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A antibody, Sema-3a antibody, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Aa antibody, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Ab antibody, SEMA3A antibody, Sema3a antibody, Sema-3a antibody, sema3aa antibody, sema3ab antibody

Background: SEMA3A is also known as COLL1, SEMA1 or SEMAL. This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.

Synonyms: Coll 1 antibody|Coll1 antibody|HH16 antibody|Hsema I antibody|Hsema III antibody|sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3A antibody|Sema III antibody|SEMA1 antibody|SEMA1-PEN antibody|SEMA3A antibody|SEMAD antibody|SEMAIII antibody|SEMAL antibody|Semaphorin 3A precursor antibody|Semaphorin III antibody|Semaphorin like growth cone guidance homolog of chick collapsin antibody|Semaphorin-3A precursor antibody|Semaphorin3A antibody|SemD antibody

Gene ID: 10371

UniProt: Q14563

Pathways: Regulation of Cell Size