LUM antibody (AA 68-338)

Catalog No. ABIN3043386

Product Details anti-LUM Antibody

Target: LUM (Lumican (LUM))

Binding Specificity: AA 68-338

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LUM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Lumican(LUM) detection. Tested with WB, IHC-P, ELISA in Mouse.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Lumican(LUM) detection. Tested with WB, IHC-P, ELISA in Mouse.
Gene Name: lumican
Protein Name: Lumican

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse Lumican recombinant protein (Position: I68-N338). Mouse Lumican shares 88.9% and 98.2% amino acid (aa) sequence identity with human and rat Lumican, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for LUM

Target: LUM (Lumican (LUM))

Alternative Name: LUM (LUM Products)

Synonyms: MGC64527 antibody, zgc:86661 antibody, ldc antibody, slrr2d antibody, DKFZp468L1116 antibody, LDC antibody, SLRR2D antibody, Ldc antibody, lumican antibody, lumican L homeolog antibody, LUM antibody, lum.L antibody, lum antibody, CpipJ_CPIJ010209 antibody, Lum antibody

Background: Lumican, also known as LUM, is an extracellular matrix protein that, in humans, is encoded by the LUM gene on chromosome 12. Lumican is a major keratan sulfate proteoglycan of the cornea but is ubiquitously distributed in most mesenchymal tissues throughout the body. And Lumican is involved in collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. Corneal transparency is possible due to the exact alignment of collagen fibers by lumican (and keratocan) in the intrafibrillar space.

Synonyms: Keratan sulfate proteoglycan lumican antibody|KSPG lumican antibody|LDC antibody|LUM antibody|LUM_HUMAN antibody|Lumican antibody| Lumican precursor antibody|SLRR2D antibody

Gene ID: 17022

UniProt: P51885

Pathways: Glycosaminoglycan Metabolic Process