DMRT1 antibody (N-Term)

Catalog No. ABIN3043450

Product Details anti-DMRT1 Antibody

Target: DMRT1 (Doublesex and Mab-3 Related Transcription Factor 1 (DMRT1))

Binding Specificity: AA 98-128, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DMRT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Doublesex- and mab-3-related transcription factor 1(DMRT1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: RDCQCKKCNL IAERQRVMAA QVALRRQQAQ E

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Doublesex- and mab-3-related transcription factor 1(DMRT1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: doublesex and mab-3 related transcription factor 1
Protein Name: Doublesex- and mab-3-related transcription factor 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human DMRT1 (98-128aa RDCQCKKCNLIAERQRVMAAQVALRRQQAQE), different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for DMRT1

Target: DMRT1 (Doublesex and Mab-3 Related Transcription Factor 1 (DMRT1))

Alternative Name: DMRT1 (DMRT1 Products)

Synonyms: DMT1 antibody, DMRT1 antibody, zgc:136676 antibody, dmrt1 antibody, dmrt1-alpha antibody, dmt1 antibody, dmrt1-beta antibody, xdmrt1 antibody, doublesex and mab-3 related transcription factor 1 antibody, doublesex and mab-3 related transcription factor 1 L homeolog antibody, doublesex and mab-3 related transcription factor 1 S homeolog antibody, DMRT1 antibody, Dmrt1 antibody, dmrt1 antibody, dmrt1.L antibody, dmrt1.S antibody

Background: Doublesex and mab-3 related transcription factor 1, also known as DMRT1, is a protein which in humans is encoded by the DMRT1 gene. This gene is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous.

Synonyms: CT154 antibody|DM domain expressed in testis 1 antibody|DM domain expressed in testis protein 1 antibody|DM domain gene expressed in testis antibody|DMRT 1 antibody|dmrt1 antibody|DMRT1_HUMAN antibody|DMT 1 antibody|DMT1 antibody|Doublesex and mab 3 related transcription factor 1 antibody|Doublesex- and mab-3-related transcription factor 1 antibody

Gene ID: 1761

UniProt: Q9Y5R6