MAFA antibody (Middle Region)

Catalog No. ABIN3043609

Product Details anti-MAFA Antibody

Target: MAFA (Transcription Factor MafA (MAFA))

Binding Specificity: AA 136-167, Middle Region

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAFA antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Transcription factor MafA(MAFA) detection. Tested with WB in Human,Rat.

Sequence: EDAVEALIGS GHHGAHHGAH HPAAAAAYEA FR

Cross-Reactivity (Details): Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Transcription factor MafA(MAFA) detection. Tested with WB in Human,Rat.
Gene Name: v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A
Protein Name: Transcription factor MafA

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human MAFA(136-167aa EDAVEALIGSGHHGAHHGAHHPAAAAAYEAFR), identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, The detection limit for MAFA is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for MAFA

Target: MAFA (Transcription Factor MafA (MAFA))

Alternative Name: MAFA (MAFA Products)

Synonyms: RIPE3b1 antibody, hMafA antibody, RGD1562627 antibody, 2F1-Ag antibody, MAFA antibody, MAFA-L antibody, SMaf1 antibody, mafl antibody, L-MAF antibody, MAFL antibody, Maf antibody, v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) antibody, MAF bZIP transcription factor A antibody, killer cell lectin-like receptor subfamily G, member 1 antibody, v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A antibody, v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog Aa antibody, monocyte to macrophage differentiation-associated antibody, Mafa antibody, MAFA antibody, Klrg1 antibody, mafa antibody, mafaa antibody, Mmd antibody

Background: MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene. MAFA plays a key role in coordinating and controlling the level of insulin gene expression in islet beta cells. It is located in 8q24.3. The intracellular domain of MAFA contains a single immunoreceptor tyrosine-based inhibitory motif (ITIM), which classifies MAFA as a member of an inhibitory receptor superfamily. The inhibitory capacity of MAFA is best defined in mast cells, where MAFA keeps in check the antigen-induced (i.e. Fc epsilon RI-induced) secretion ofinflammatory mediators.

Synonyms: HMafA antibody|Mafa antibody|MAFA_HUMAN antibody|Pancreatic beta cell specific transcriptional activator antibody|Pancreatic beta-cell-specific transcriptional activator antibody|RIPE3b1 antibody|Transcription factor MafA antibody|Transcription factor RIPE3b1 antibody|v maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian) antibody|v maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody|V-maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody

Gene ID: 389692

Pathways: Hormone Transport