Paxillin antibody (PXN) (AA 305-559)

Details for Product anti-PXN Antibody No. ABIN3043666, Supplier: Log in to see
Antigen
  • AW108311
  • AW123232
  • Pax
  • CG18061
  • CG18576
  • CG31794
  • CT40481
  • CT42454
  • DPaxillin
  • DPxn
  • DPxn37
  • Dmel\\CG31794
  • Dpax
  • DpaxA
  • PDLP
  • dPax
  • pax
  • wu:fw71f12
  • PXN
  • LOC100220858
  • paxillin
  • Paxillin
  • paxillin S homeolog
  • paxillin a
  • PXN
  • Pxn
  • Pax
  • pxn.S
  • pxna
  • LOC696336
  • pxn
Alternatives
anti-Human Paxillin antibody for Western Blotting
Epitope
AA 305-559, C-Term
59
55
30
25
23
15
13
12
11
10
8
7
6
6
6
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
403
214
203
24
12
10
8
6
5
5
5
3
1
1
1
1
1
Host
Mouse
375
36
2
Clonality (Clone)
Monoclonal ()
Conjugate
This Paxillin antibody is un-conjugated
7
7
7
5
5
5
5
5
5
5
5
5
4
4
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Western Blotting (WB)
334
126
110
64
52
44
26
20
11
8
4
2
1
1
1
Options
Supplier
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Supplier Product No.
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Purpose Mouse IgG monoclonal antibody for Paxillin, paxillin (PXN) detection. Tested with WB, ICC in Human,mouse,rat,chicken. No cross reactivity with other proteins.
Immunogen C-terminal part of recombinant chicken paxillin(amino acids 305-559).
Clone PXC-10
Isotype IgG1
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Mouse IgG monoclonal antibody for Paxillin, paxillin (PXN) detection. Tested with WB, ICC in Human,mouse,rat,chicken.
Gene Name: paxillin
Protein Name: Paxillin
Purification Ascites
Plasmids, Primers & others Plasmids, Primers & others Paxillin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name PXN (PXN Antibody Abstract)
Background The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68- kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein(paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney(MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.
Gene ID 5829, 19303, 360820
UniProt P49023, Q66H76
Pathways MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
Application Notes WB: Concentration: 1-2 μg/mL, Species: Human, mouse, rat, chicken
ICC: Concentration: 1 μg/mL, Species: Human, mouse, rat, chicken
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921123 in WB, supported by ABIN921230 in ICC.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 1 mL of PBS buffer will yield a concentration of 100 μg/mL.
Concentration 100 μg/mL
Buffer Mouse ascites fluid, 1.2 % sodium acetate, 2 mg BSA, with 0.01 mg Sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Supplier Images
Western Blotting (WB) image for anti-Paxillin (PXN) (AA 305-559), (C-Term) antibody (ABIN3043666) Anti-Paxillin antibody (monoclonal), Western blotting Lane 1: 293T Cell Lysate Lane 2...
Product cited in: Zhu, Liu, Li, Liu, Wang, Sun, Xiong, Jiang, Zheng, Hu: "Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility." in: Carcinogenesis, Vol. 35, Issue 8, pp. 1901-10, 2014 (PubMed).

Liu, Zhu, Xiong, Zheng, Hu, Qiu: "Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells." in: International journal of clinical and experimental pathology, Vol. 7, Issue 9, pp. 5750-61, 2014 (PubMed).

Zhong, Luo, Wang, Tang, Wu, Wang, Huang, Sun, Huang: "Effects of polydopamine functionalized titanium dioxide nanotubes on endothelial cell and smooth muscle cell." in: Colloids and surfaces. B, Biointerfaces, Vol. 116, pp. 553-60, 2014 (PubMed).

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