BRAF antibody (AA 38-230)

Catalog No. ABIN3043761

Product Details anti-BRAF Antibody

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Binding Specificity: AA 38-230

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase B-raf(BRAF) detection. Tested with WB in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase B-raf(BRAF) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: B-Raf proto-oncogene, serine/threonine kinase
Protein Name: Serine/threonine-protein kinase B-raf

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human B Raf recombinant protein (Position: A38-V230). Human B Raf shares 81% amino acid (aa) sequence identity with mouse B Raf.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for B Raf is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF (BRAF Products)

Synonyms: B-RAF1 antibody, BRAF1 antibody, NS7 antibody, RAFB1 antibody, C-RMIL antibody, RMIL antibody, B-Raf antibody, braf1 antibody, rafb1 antibody, b-raf1 antibody, sierrabraf antibody, BRAF antibody, 9930012E13Rik antibody, AA120551 antibody, AA387315 antibody, AA473386 antibody, B-raf antibody, Braf-2 antibody, Braf2 antibody, C230098H17 antibody, C87398 antibody, D6Ertd631e antibody, braf antibody, fc48g01 antibody, wu:fc48g01 antibody, B-Raf proto-oncogene, serine/threonine kinase antibody, B-Raf proto-oncogene, serine/threonine kinase S homeolog antibody, Braf transforming gene antibody, si:dkey-222f8.3 antibody, BRAF antibody, Braf antibody, braf.S antibody, braf antibody, si:dkey-222f8.3 antibody

Background: BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf. It is a member of the Raf kinase family of growth signal transduction protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. It is mapped to 7q34. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. The BRAF protein is also involved in sending signals inside cells, which are involved in directing cell growth.

Synonyms: FLJ95109 antibody|94 kDa B raf protein antibody|B raf 1 antibody|B raf 1 antibody|B Raf proto oncogene serine threonine protein kinase antibody|B Raf proto oncogene serine threonine protein kinase antibody|B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody|BRAF 1 antibody|BRaf antibody|BRaf antibody|BRAF_HUMAN antibody|BRAF1 antibody|BRAF1 antibody|cRmil antibody|MGC126806 antibody|MGC138284 antibody|Murine sarcoma viral (v-raf) oncogene homolog B1 antibody|Murine sarcoma viral (v-raf) oncogene homolog B1 antibody|Murine sarcoma viral v raf oncogene homolog B1 antibody|Murine sarcoma viral v raf oncogene homolog B1 antibody|NS7 antibody|oncogene BRAF1 antibody|p94 antibody|p94 antibody|Proto-oncogene B-Raf antibody|Proto-oncogene c-Rmil antibody|RAFB 1 antibody|RAFB1 antibody|RAFB1 antibody|RMIL antibody|Serine/threonine-protein kinase B-raf antibody|v raf murine sarcoma viral oncogene homolog B antibody|v raf murine sarcoma viral oncogene homolog B1 antibody|v raf murine sarcoma viral oncogene homolog B1 antibody|v-Raf murine sarcoma viral oncogene homolog B1 antibody

Gene ID: 673

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy